Transgenic expression of cif genes from Wolbachia strain wAlbB recapitulates cytoplasmic incompatibility in Aedes aegypti.
Transgenic expression of cif genes from Wolbachia strain wAlbB recapitulates cytoplasmic incompatibility in Aedes aegypti.
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Wolbachia菌株wAlbB中cif基因的转基因表达再现了埃及伊蚊细胞质的不亲和性。
DOI：
10.1038/s41467-024-45238-7
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发表时间：
2024-01-29
影响因子：
16.6
通讯作者：
Sinkins, Steven P.
中科院分区：
文献类型：
--
作者：
Mcnamara, Cameron J.;Ant, Thomas H.;Harvey-Samuel, Tim;White-Cooper, Helen;Martinez, Julien;Alphey, Luke;Sinkins, Steven P.
内共生细菌沃尔巴克氏体可以通过细胞质不相容性（CI）改变宿主繁殖来入侵昆虫种群，当携带沃尔巴克氏体的雄性与无沃尔巴克氏体的雌性交配时，这种效应会导致胚胎死亡。在这里，我们描述了一种转基因系统，用于重建CI的主要虫媒病毒载体埃及伊蚊使用CI因子（CIF）基因wAlbB，沃尔巴克氏体菌株目前正在部署，以减少登革热传播。当cifA和cifB在睾丸中共表达时，诱导CI样不育;这种不育通过母体cifA表达来挽救，从而再现沃尔巴克氏体诱导的CI的模式。cifB单独表达与广泛的DNA损伤和精子发生中断有关。母体cifA表达的拯救强度取决于雄性中cifA/cifB表达的相对水平。这些发现与CifB作为毒素和CifA作为抗毒素一致，CifA减弱CifB在雄性生殖系和发育中的胚胎中的毒性。这些发现为深入研究cif基因之间的相互作用及其活性机制提供了重要的信息，并为构建Ae中基于cif基因的驱动系统奠定了基础。埃及人。Wolbachia cifA和cifB基因在昆虫宿主中产生细胞质不相容性（CI），但cifA的作用仍有争议。在这里，作者报告了CI在主要虫媒病毒载体埃及伊蚊中的转基因重现，并提供了cifA抑制cifB在雄性生殖系中毒性的证据。
The endosymbiotic bacteria Wolbachia can invade insect populations by modifying host reproduction through cytoplasmic incompatibility (CI), an effect that results in embryonic lethality when Wolbachia-carrying males mate with Wolbachia-free females. Here we describe a transgenic system for recreating CI in the major arbovirus vector Aedes aegypti using CI factor (cif) genes from wAlbB, a Wolbachia strain currently being deployed to reduce dengue transmission. CI-like sterility is induced when cifA and cifB are co-expressed in testes; this sterility is rescued by maternal cifA expression, thereby reproducing the pattern of Wolbachia-induced CI. Expression of cifB alone is associated with extensive DNA damage and disrupted spermatogenesis. The strength of rescue by maternal cifA expression is dependent on the comparative levels of cifA/cifB expression in males. These findings are consistent with CifB acting as a toxin and CifA as an antitoxin, with CifA attenuating CifB toxicity in both the male germline and in developing embryos. These findings provide important insights into the interactions between cif genes and their mechanism of activity and provide a foundation for the building of a cif gene-based drive system in Ae. aegypti. The Wolbachia cifA and cifB genes generate cytoplasmic incompatibility (CI) in insect hosts but the role of cifA is still debated. Here, the authors report the transgenic recapitulation of CI in the major arbovirus vector Aedes aegypti and provide evidence for cifA inhibiting cifB toxicity in the male germline.
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影响因子：
3.8
作者：
Liu WL;Yu HY;Chen YX;Chen BY;Leaw SN;Lin CH;Su MP;Tsai LS;Chen Y;Shiao SH;Xi Z;Jang AC;Chen CH
通讯作者：
Chen CH
Culex Pipiens越过类型多样性受Wolbachia的扩增和多态性操纵子的控制。
影响因子：
16.6
作者：
Bonneau M;Atyame C;Beji M;Justy F;Cohen-Gonsaud M;Sicard M;Weill M
通讯作者：
Weill M
WOLBACHIA菌株WAU在埃及埃及伊德斯（Aedes Aegypti）中提供了高效的病毒传递阻滞。
影响因子：
6.7
作者：
Ant TH;Herd CS;Geoghegan V;Hoffmann AA;Sinkins SP
通讯作者：
Sinkins SP
释放不兼容的男性在澳大利亚的野生和沃尔巴契亚岛伊德斯埃及埃及埃及的人群中进行了强烈的抑制。
DOI：
10.1073/pnas.2106828118
发表时间：
2021-10-12
影响因子：
11.1
作者：
Beebe NW;Pagendam D;Trewin BJ;Boomer A;Bradford M;Ford A;Liddington C;Bondarenco A;De Barro PJ;Gilchrist J;Paton C;Staunton KM;Johnson B;Maynard AJ;Devine GJ;Hugo LE;Rasic G;Cook H;Massaro P;Snoad N;Crawford JE;White BJ;Xi Z;Ritchie SA
通讯作者：
Ritchie SA
影响因子：
16.6
作者：
Anderson, Michelle A. E.;Gonzalez, Estela;Ang, Joshua X. D.;Shackleford, Lewis;Nevard, Katherine;Verkuijl, Sebald A. N.;Edgington, Matthew P. P.;Harvey-Samuel, Tim;Alphey, Luke
通讯作者：
Alphey, Luke