Lab-scale characterization and semi-field trials of Wolbachia Strain wAlbB in a Taiwan Wolbachia introgressed Ae. aegypti strain.
Lab-scale characterization and semi-field trials of Wolbachia Strain wAlbB in a Taiwan Wolbachia introgressed Ae. aegypti strain.
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DOI：
10.1371/journal.pntd.0010084
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发表时间：
2022-01
影响因子：
3.8
通讯作者：
Chen CH
中科院分区：
文献类型：
--
作者：
Liu WL;Yu HY;Chen YX;Chen BY;Leaw SN;Lin CH;Su MP;Tsai LS;Chen Y;Shiao SH;Xi Z;Jang AC;Chen CH
登革热是由伊蚊传播的最严重的病毒性疾病之一，传统的疾病控制方法不足以预防重大疾病负担。释放Wolbachia transinfected蚊子提供了一个有前途的替代控制方法; Wolbachia transinfected女性埃及伊蚊表现出减少登革热病毒的传播，而Wolbachia transinfected男性造成合子致死时，与未感染的女性，提供了一种方法，抑制蚊子种群。虽然非常有希望，人口控制策略的微妙性质和当地物种种群之间的差异意味着，如果不对特定的当地Ae进行广泛的测试，就不能控制释放Wolbachia感染的蚊子。埃及人。为了研究利用Wolbachia抑制局部Ae的潜力。埃及种群在台湾，我们进行了实验室为基础的和半场健身试验。我们首先将Wolbachia菌株wAlbB转染到当地的Ae中。埃及种群（wAlbB-Tw），并发现与对照组相比，寿命，繁殖力和生育力没有显着变化。在实验室中，我们发现随着释放的携带沃尔巴克氏体的雄蚊比例的增加，种群抑制可以达到100%。半场实验中的等效实验发现抑制率高达70%。在半场系统中释放不同比例的wAlbB-Tw雄性提供了雄性释放的最佳大小的估计。我们的研究结果表明，wAlbB-Tw具有显着的潜力，用于针对Ae的载体控制策略。台湾的埃及人人口减少。现在有必要进行旷场释放试验以确认wAlbB-Tw介导的抑制在自然环境中是可行的。在野外释放之前，干扰蚊子能力和降低蚊子种群密度的新遗传方法需要在实验室和田间试验中进行逐步评估。因此，在封闭的室外系统中进行试验是重要的一步，可以在包含自然环境变化的环境中评估受沃尔巴克氏体感染的埃及伊蚊（wAlbB-Tw）的有效性和可靠性。我们描述了一个半场系统，旨在研究wAlbB-Tw蚊子抑制当地蚊子种群的能力。我们提供了一个建立，维护和监测Ae的协议。田间笼内的埃及种群密度。这些结果为wAlbB-Tw感染的Ae的开放释放铺平了道路。埃及蚊子在田野里
Dengue fever is one of the most severe viral diseases transmitted by Aedes mosquitoes, with traditional approaches of disease control proving insufficient to prevent significant disease burden. Release of Wolbachia-transinfected mosquitoes offers a promising alternative control methodologies; Wolbachia-transinfected female Aedes aegypti demonstrate reduced dengue virus transmission, whilst Wolbachia-transinfected males cause zygotic lethality when crossed with uninfected females, providing a method for suppressing mosquito populations. Although highly promising, the delicate nature of population control strategies and differences between local species populations means that controlled releases of Wolbachia-transinfected mosquitoes cannot be performed without extensive testing on specific local Ae. aegypti populations. In order to investigate the potential for using Wolbachia to suppress local Ae. aegypti populations in Taiwan, we performed lab-based and semi-field fitness trials. We first transinfected the Wolbachia strain wAlbB into a local Ae. aegypti population (wAlbB-Tw) and found no significant changes in lifespan, fecundity and fertility when compared to controls. In the laboratory, we found that as the proportion of released male mosquitoes carrying Wolbachia was increased, population suppression could reach up to 100%. Equivalent experiments in semi-field experiments found suppression rates of up to 70%. The release of different ratios of wAlbB-Tw males in the semi-field system provided an estimate of the optimal size of male releases. Our results indicate that wAlbB-Tw has significant potential for use in vector control strategies aimed at Ae. aegypti population suppression in Taiwan. Open field release trials are now necessary to confirm that wAlbB-Tw mediated suppression is feasible in natural environments. Prior to open field release, new genetic approaches that interfere with mosquito abilities and reduce mosquito population density require progressive evaluation both in the laboratory and contained field trials. Trials in contained outdoor systems are thus an important step and allow for evaluation of the effectiveness and reliability of Wolbachia-infected Aedes aegypti (wAlbB-Tw) in settings that incorporate natural environmental variations. We describe a semi-field system designed to study the ability of wAlbB-Tw mosquitoes to suppress local mosquito populations. We provide a protocol for establishing, maintaining, and monitoring Ae. aegypti population densities inside field cages. These results pave the way for the open release of wAlbB-Tw-infected Ae. aegypti mosquitoes in the field.
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影响因子：
3.8
作者：
Frentiu FD;Zakir T;Walker T;Popovici J;Pyke AT;van den Hurk A;McGraw EA;O'Neill SL
通讯作者：
O'Neill SL
影响因子：
2.1
作者：
Calvitti, Maurizio;Moretti, Riccardo;Dobson, Stephen L.
通讯作者：
Dobson, Stephen L.
影响因子：
3.2
作者：
Brelsfoard CL;Dobson SL
通讯作者：
Dobson SL
影响因子：
3.8
作者：
Hoffmann AA;Iturbe-Ormaetxe I;Callahan AG;Phillips BL;Billington K;Axford JK;Montgomery B;Turley AP;O'Neill SL
通讯作者：
O'Neill SL
影响因子：
4.1
作者：
Hoffmann AA;Ross PA;Rašić G
通讯作者：
Rašić G