OsKinesin-13A is an active microtubule depolymerase involved in glume length regulation via affecting cell elongation.
OsKinesin-13A is an active microtubule depolymerase involved in glume length regulation via affecting cell elongation.
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DOI：
10.1038/srep09457
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发表时间：
2015-03-25
影响因子：
4.6
通讯作者：
Wang T
中科院分区：
文献类型：
--
作者：
Deng ZY;Liu LT;Li T;Yan S;Kuang BJ;Huang SJ;Yan CJ;Wang T
籽粒大小是影响水稻产量和品质的重要性状，决定籽粒大小的主要因素是颖壳大小。然而，颖壳大小是如何调节的在很大程度上仍是个未知数。在这里，我们报告了OsKinesin-13A的特征，它调节水稻细胞的伸长和颖片长度。OsKinesin-13A突变体Sar1在籽粒和节间、叶片和根等器官中表现出长度缩短。Sar1的籽粒表型是颖片长度减少直接导致的，颖片长度的减少限制了颖果的大小。组织学结果显示，细胞伸长的异常导致了sr1器官的长度缩短。在SAR1中，纤维微原纤维的取向存在缺陷。一贯地，sr1显示皮质微管的横向取向减少。进一步的观察表明，sr1的微管周转率降低。OsKinesin-13A是一种活性微管解聚酶，主要分布在高尔基体的囊泡上，定位于细胞表面。因此，我们的结果表明OsKinesin-13A利用其微管解聚活性来促进微管翻转，这不仅可能影响皮质微管的横向取向，还可能促进囊泡从高尔基体到细胞表面的运输，从而影响纤维素微纤维的取向和细胞的伸长。
Grain size is an important trait influencing both the yield and quality of rice and its major determinant is glume size. However, how glume size is regulated remains largely unknown. Here, we report the characterization of OsKinesin-13A, which regulates cell elongation and glume length in rice. The mutant of OsKinesin-13A, sar1, displayed length reduction in grains and other organs including internodes, leaves and roots. The grain phenotype in sar1 was directly caused by reduction in glume length, which in turn restricted caryopsis size. Histological results revealed that length decrease in sar1 organs resulted from abnormalities in cell elongation. The orientation of cellulose microfibrils was defective in sar1. Consistently, sar1 showed reduced transverse orientation of cortical microtubules. Further observations demonstrated that microtubule turnover was decreased in sar1. OsKinesin-13A was shown to be an active microtubule depolymerase and mainly distributed on vesicles derived from the Golgi apparatus and destined for the cell surface. Thus, our results suggest that OsKinesin-13A utilizes its microtubule depolymerization activity to promote microtubule turnover, which may not only influence transverse orientation of cortical microtubules but also facilitate vesicle transport from the Golgi apparatus to the cell surface, and thus affects cellulose microfibril orientation and cell elongation.
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