Development of an apparatus and methodology for conducting functional magnetic resonance imaging (fMRI) with pharmacological stimuli in conscious rhesus monkeys.
Development of an apparatus and methodology for conducting functional magnetic resonance imaging (fMRI) with pharmacological stimuli in conscious rhesus monkeys.
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DOI：
10.1016/j.jneumeth.2010.06.001
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发表时间：
2010-08-15
影响因子：
3
通讯作者：
Howell, Leonard Lee
中科院分区：
文献类型：
--
作者：
Murnane, Kevin Sean;Howell, Leonard Lee
功能性磁共振成像（fMRI）是一种具有重要潜力的技术，以促进我们对多个大脑系统的理解。然而，当人类受试者接受功能性磁共振成像研究时，他们通常是有意识的，而临床前功能性磁共振成像研究通常使用麻醉，这使得研究之间的比较复杂化。因此，我们已经开发了一种设备，适用于成像有意识的恒河猴。为了尽量减少受试者的压力和空间运动，每名受试者在几个月内适应必要的程序。然后，通过量化客观的生理测量，在经过充分训练的受试者中评估该过程的有效性。这些生理指标在会话内和会话间都是稳定的，并且与使用标准灵长类动物处理程序固定这些相同受试者时没有差异。然后通过在三种不同条件下扫描受试者来评估受试者运动和血氧水平依赖（BOLD）fMRI测量结果：无刺激、视觉刺激或静脉内（i. v.）可卡因（0.3毫克/千克）。空间运动既不因条件而异，也不沿沿着而异。此外，最大平移和旋转运动从未超过体素尺寸的一半（0.75 mm）或1.5度。此外，血氧变化的定位与以前使用类似刺激的研究中报道的密切匹配。这些发现证明了使用这些程序在有意识的恒河猴中进行fMRI数据收集的可行性，并允许进一步研究精神药物的神经效应。
Functional magnetic resonance imaging (fMRI) is a technique with significant potential to advance our understanding of multiple brain systems. However, when human subjects undergo fMRI studies they are typically conscious whereas pre-clinical fMRI studies typically utilize anesthesia, which complicates comparisons across studies. Therefore, we have developed an apparatus suitable for imaging conscious rhesus monkeys. In order to minimize subject stress and spatial motion, each subject was acclimated to the necessary procedures over several months. The effectiveness of this process was then evaluated, in fully trained subjects, by quantifying objective physiological measures. These physiological metrics were stable both within and across sessions and did not differ from when these same subjects were immobilized using standard primate handling procedures. Subject motion and blood oxygenation level dependent (BOLD) fMRI measurements were then evaluated by scanning subjects under three different conditions: the absence of stimulation, presentation of a visual stimulus, or administration of intravenous (i.v.) cocaine (0.3 mg/kg). Spatial motion differed neither by condition nor along the three principal axes. In addition, maximum translational and rotational motion never exceeded one half of the voxel size (0.75 mm) or 1.5 degrees, respectively. Furthermore, the localization of changes in blood oxygenation closely matched those reported in previous studies using similar stimuli. These findings document the feasibility of fMRI data collection in conscious rhesus monkeys using these procedures and allow for the further study of the neural effects of psychoactive drugs.
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影响因子：
3
作者：
Howell, LL;Hoffman, JM;Jordan, JF
通讯作者：
Jordan, JF
DOI：
10.1007/bf03206665
发表时间：
2005-04-01
期刊：
NeuroRx : the journal of the American Society for Experimental NeuroTherapeutics
影响因子：
--
作者：
Bammer, Roland;Skare, Stefan;Glover, Gary H
通讯作者：
Glover, Gary H
影响因子：
25
作者：
Logothetis, NK;Guggenberger, H;Pauls, J
通讯作者：
Pauls, J
影响因子：
5.7
作者：
Kufahl, PR;Li, Z;Li, SJ
通讯作者：
Li, SJ
影响因子：
4.8
作者：
Gamlin, PD;Ward, MK;Twieg, DB
通讯作者：
Twieg, DB