Comparison of Radical Scavenging Activity, Cytotoxic Effects and Apoptosis Induction in Human Melanoma Cells by Taiwanese Propolis from Different Sources.
Comparison of Radical Scavenging Activity, Cytotoxic Effects and Apoptosis Induction in Human Melanoma Cells by Taiwanese Propolis from Different Sources.
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DOI：
10.1093/ecam/neh034
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发表时间：
2004-09-01
期刊：
影响因子：
--
通讯作者：
Lin JK
中科院分区：
文献类型：
--
作者：
Chen CN;Weng MS;Wu CL;Lin JK
蜂胶是蜜蜂从植物中采集的粘性物质。我们先前研究发现，从台湾红豆杉中分离出的propolins A、B、C、D、E和F能有效地诱导人黑色素瘤细胞凋亡，并且是强抗氧化剂。在这项研究中，我们评价TP清除自由基的DPPH（1，2-二苯基-2-苦肼基）的活性。酚类浓度通过Folin-Ciocalteu方法定量。评价了人黑素瘤细胞中的凋亡触发活性。茶多酚中酚类物质含量较高，具有较强的清除自由基的能力。此外，TP 1g、TP 3、TP 4和TP 7对人黑色素瘤细胞具有细胞毒性作用，IC 50分别为2.3、2.0、3.3和3.3 μg/ml。流式细胞仪DNA片段分析表明，TP 1g，TP 2，TP 3和TP 7可以诱导人黑素瘤细胞凋亡，并且细胞从细胞周期的G2/M期有明显的损失。为了阐明TP的凋亡作用机制，我们评价了TP对人黑素瘤细胞凋亡相关蛋白的诱导作用。半胱天冬酶原-3和PARP [聚（ADP-核糖）聚合酶]水平显着降低。此外，还采用HPLC法测定了茶多酚中的蜂胶A、B、C、D、E和F。结果表明，TP是这些化合物的丰富来源。研究结果表明，TP诱导人黑色素瘤细胞凋亡，由于其高水平的propolins。
Propolis is a sticky substance that is collected from plants by honeybees. We previously demonstrated that propolins A, B, C, D, E and F, isolated from Taiwanese propolis (TP), could effectively induce human melanoma cell apoptosis and were strong antioxidant agents. In this study, we evaluated TP for free radical scavenging activity by DPPH (1,2-diphenyl-2-picrylhydrazyl). The phenolic concentrations were quantified by the Folin–Ciocalteu method. The apoptosis trigger activity in human melanoma cells was evaluated. TP contained a higher level of phenolic compounds and showed strong capability to scavenge free radicals. Additionally, TP1g, TP3, TP4 and TP7 exhibited a cytotoxic effect on human melanoma cells, with an IC50 of ∼2.3, 2.0, 3.3 and 3.3 μg/ml, respectively. Flow cytometric analysis for DNA fragmentation indicated that TP1g, TP2, TP3 and TP7 could induce apoptosis in human melanoma cells and there is a marked loss of cells from the G2/M phase of the cell cycle. To address the mechanism of the apoptosis effect of TP, we evaluated its effects on induction of apoptosis-related proteins in human melanoma cells. The levels of procaspase-3 and PARP [poly(ADP-ribose) polymerase] were markedly decreased. Furthermore, propolins A, B, C, D, E and F in TP were determined using HPLC. The results indicate that TP is a rich source of these compounds. The findings suggest that TP induces apoptosis in human melanoma cells due to its high level of propolins.
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