Changes in Gut Microorganism in Patients with Positive Immune Antibody-Associated Recurrent Abortion.
Changes in Gut Microorganism in Patients with Positive Immune Antibody-Associated Recurrent Abortion.
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免疫抗体阳性相关复发性流产患者肠道微生物的变化
DOI：
10.1155/2020/4673250
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发表时间：
2020
影响因子：
--
通讯作者：
Feng X
中科院分区：
文献类型：
--
作者：
Jin M;Li D;Ji R;Liu W;Xu X;Feng X
本研究旨在利用16s rRNA基因测序微生物组法分析免疫抗体阳性的复发性流产患者肠道微生物的变化。收集20例免疫抗体阳性（阳性组）和20例免疫抗体阴性（阴性组）的反复流产妇女的粪便样本。16s rRNA基因测序后，对得到的原始reads进行高质量过滤，获得干净的标签，然后分类到微生物基因组中。将所有有效标签聚类为操作分类单元（operational taxonomic units, otu），选取具有代表性的序列进行分类信息标注，并进行alpha和beta多样性分析。40份样本共获得43,116个otu。阳性组拟杆菌的相对丰度最高。阴性组中Bacteroides、丹毒三甲杆菌ucg -003、Faecalibacterium和Prevotella_9相对丰度较高。α多样性分析结果显示，阳性处理组的群落丰富度、群落多样性和系统发育多样性均高于阴性处理组。普雷沃氏菌、肠球菌、巨球菌和厌氧菌在阴性组和阳性组间差异有统计学意义。本研究首次通过16s rRNA基因测序微生物组测定研究了与阳性免疫抗体相关的复发性流产相关的肠道微生物组。阳性组和阴性组之间有显著差异的属可能作为阳性免疫抗体相关的复发性流产的治疗靶点。
This study is aimed at analyzing the changes in gut microorganism of patients with positive immune antibody-associated recurrent abortion using the 16s rRNA gene sequencing microbiome assay. The fecal samples from 20 recurrent abortion women with positive immune antibody (positive group) and 20 with negative immune antibody (negative group) were collected. After 16s rRNA gene sequencing, the obtained raw reads underwent quality filtering to obtain the clean tags and then classified into microbial genomes. All effective tags were clustered into operational taxonomic units (OTUs), and the representative sequence was selected for the annotation of taxonomic information, followed by alpha and beta diversity analyses. A total of 43,116 OTUs were obtained in all 40 samples. Bacteroides had the highest relative abundance in the positive group. In the negative group, Bacteroides, Erysipelotrichaceae_UCG-003, Faecalibacterium, and Prevotella_9 had high relative abundance. Alpha diversity analysis results showed that the community richness, community diversity, and phylogenetic diversity in the positive group were higher than that in the negative group. Prevotella_9, Enterococcus, Megasphaera, and Anaerostipes presented significant differences between negative and positive groups. The present study for the first time investigated the gut microbiome involved in positive immune antibody-associated recurrent abortion via the 16s rRNA gene sequencing microbiome assay. The genera that were significantly differential between positive and negative groups may serve as therapeutic targets for positive immune antibody-associated recurrent abortion.
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影响因子：
64.8
作者：
Arumugam, Manimozhiyan;Raes, Jeroen;Pelletier, Eric;Le Paslier, Denis;Yamada, Takuji;Mende, Daniel R.;Fernandes, Gabriel R.;Tap, Julien;Bruls, Thomas;Batto, Jean-Michel;Bertalan, Marcelo;Borruel, Natalia;Casellas, Francesc;Fernandez, Leyden;Gautier, Laurent;Hansen, Torben;Hattori, Masahira;Hayashi, Tetsuya;Kleerebezem, Michiel;Kurokawa, Ken;Leclerc, Marion;Levenez, Florence;Manichanh, Chaysavanh;Nielsen, H. Bjorn;Nielsen, Trine;Pons, Nicolas;Poulain, Julie;Qin, Junjie;Sicheritz-Ponten, Thomas;Tims, Sebastian;Torrents, David;Ugarte, Edgardo;Zoetendal, Erwin G.;Wang, Jun;Guarner, Francisco;Pedersen, Oluf;de Vos, Willem M.;Brunak, Soren;Dore, Joel;Weissenbach, Jean;Ehrlich, S. Dusko;Bork, Peer
通讯作者：
Bork, Peer
影响因子：
2.9
作者：
Egshatyan L;Kashtanova D;Popenko A;Tkacheva O;Tyakht A;Alexeev D;Karamnova N;Kostryukova E;Babenko V;Vakhitova M;Boytsov S
通讯作者：
Boytsov S
影响因子：
2.3
作者：
Allen-Vercoe, Emma;Daigneault, Michelle;Lawson, Paul A.
通讯作者：
Lawson, Paul A.
影响因子：
3.7
作者：
Holodniy, Mark;Brown, Sheldon T.;Darbyshire, Janet
通讯作者：
Darbyshire, Janet
影响因子：
6.1
作者：
Lashen, H;Fear, K;Sturdee, DW
通讯作者：
Sturdee, DW