Place Cells in Head-Fixed Mice Navigating a Floating Real-World Environment.
Place Cells in Head-Fixed Mice Navigating a Floating Real-World Environment.
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DOI：
10.3389/fncel.2021.618658
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发表时间：
2021
影响因子：
5.3
通讯作者：
Schultz SR
中科院分区：
文献类型：
--
作者：
Go MA;Rogers J;Gava GP;Davey CE;Prado S;Liu Y;Schultz SR
啮齿动物的海马位置细胞系统为记忆功能和功能障碍的科学研究提供了一个主要范例。在自由移动的动物和在虚拟现实环境中导航的头部固定动物的海马 CA1 区中都观察到了位置细胞。然而，虚拟现实准备中的空间编码已被观察到受到损害。在这里，我们展示了使用头部固定小鼠的真实世界环境系统（由带有近端线索的空中漂浮轨道组成）在空间记忆研究方面比虚拟现实系统具有一些优势。我们对注射了基因编码钙指示剂 GCaMP6 的头部固定小鼠的海马体进行了成像，同时它们在浮动平台上的循环受限或开放环境中导航。尽管没有远端视觉线索，但我们在相当一部分细胞中观察到一致的位置调谐。当动物进入不同的环境时，重新映射区域。当动物重新进入相同的环境时，地点场通常会在几天的时间内重新映射，比自由移动的准备更快，但与虚拟现实相当。空间信息率在自由活动的小鼠中观察到的范围内。流形分析表明，可以从神经群体动态的低维子空间中提取空间信息。这是首次演示头部固定小鼠的位置细胞在空中升降的现实世界平台上导航，验证了其在研究涉及记忆和受神经退行性疾病影响的大脑回路中的用途。
The hippocampal place cell system in rodents has provided a major paradigm for the scientific investigation of memory function and dysfunction. Place cells have been observed in area CA1 of the hippocampus of both freely moving animals, and of head-fixed animals navigating in virtual reality environments. However, spatial coding in virtual reality preparations has been observed to be impaired. Here we show that the use of a real-world environment system for head-fixed mice, consisting of an air-floating track with proximal cues, provides some advantages over virtual reality systems for the study of spatial memory. We imaged the hippocampus of head-fixed mice injected with the genetically encoded calcium indicator GCaMP6s while they navigated circularly constrained or open environments on the floating platform. We observed consistent place tuning in a substantial fraction of cells despite the absence of distal visual cues. Place fields remapped when animals entered a different environment. When animals re-entered the same environment, place fields typically remapped over a time period of multiple days, faster than in freely moving preparations, but comparable with virtual reality. Spatial information rates were within the range observed in freely moving mice. Manifold analysis indicated that spatial information could be extracted from a low-dimensional subspace of the neural population dynamics. This is the first demonstration of place cells in head-fixed mice navigating on an air-lifted real-world platform, validating its use for the study of brain circuits involved in memory and affected by neurodegenerative disorders.
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影响因子：
16.2
作者：
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