Volatile-mediated interactions between phylogenetically different soil bacteria.
Volatile-mediated interactions between phylogenetically different soil bacteria.
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DOI：
10.3389/fmicb.2014.00289
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发表时间：
2014
影响因子：
5.2
通讯作者：
de Boer W
中科院分区：
文献类型：
--
作者：
Garbeva P;Hordijk C;Gerards S;de Boer W
越来越多的证据表明，有机挥发物在多孔土壤基质中微生物之间的相互作用中起着重要作用。在这里，我们报告了不同土壤细菌释放的挥发性化合物可以影响土壤细菌荧光假单胞菌Pf 0 -1的生长，抗生素生产和基因表达。我们应用了一种新的培养方法，该方法模拟了土壤中的天然营养异质性，其中在营养限制琼脂上生长的荧光假单胞菌暴露于由4种遗传上不同的细菌分离株（草地粘单胞菌、Serratia muthica、类芽孢杆菌属，和土壤杆菌属（Pedobacter sp.）生长在含有人工根系分泌物的沙地上。与我们的预期相反，产生的挥发物刺激而不是抑制荧光假单胞菌的生长。基于微阵列的全基因组分析显示，所有四种细菌菌株的挥发物都会影响荧光假单胞菌的基因表达，但每种菌株的基因表达模式不同。基于不同表达基因的注释，细菌挥发物似乎诱导荧光假单胞菌的趋化运动反应，但也诱导氧化应激反应。更详细的研究表明，C.荧光假单胞菌中的抗微生物次级代谢产物。我们的研究结果表明，细菌挥发物可以有一个重要的作用，在通信，营养和拮抗作用的土壤细菌群落。
There is increasing evidence that organic volatiles play an important role in interactions between micro-organisms in the porous soil matrix. Here we report that volatile compounds emitted by different soil bacteria can affect the growth, antibiotic production and gene expression of the soil bacterium Pseudomonas fluorescens Pf0–1. We applied a novel cultivation approach that mimics the natural nutritional heterogeneity in soil in which P. fluorescens grown on nutrient-limited agar was exposed to volatiles produced by 4 phylogenetically different bacterial isolates (Collimonas pratensis, Serratia plymuthica, Paenibacillus sp., and Pedobacter sp.) growing in sand containing artificial root exudates. Contrary to our expectation, the produced volatiles stimulated rather than inhibited the growth of P. fluorescens. A genome-wide, microarray-based analysis revealed that volatiles of all four bacterial strains affected gene expression of P. fluorescens, but with a different pattern of gene expression for each strain. Based on the annotation of the differently expressed genes, bacterial volatiles appear to induce a chemotactic motility response in P. fluorescens, but also an oxidative stress response. A more detailed study revealed that volatiles produced by C. pratensis triggered, antimicrobial secondary metabolite production in P. fluorescens. Our results indicate that bacterial volatiles can have an important role in communication, trophic - and antagonistic interactions within the soil bacterial community.
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影响因子：
2.8
作者：
Kai, Marco;Effmert, Uta;Piechulla, Birgit
通讯作者：
Piechulla, Birgit
影响因子：
9.7
作者：
Gu, Ying-Qi;Mo, Ming-He;Zhang, Ke-Qin
通讯作者：
Zhang, Ke-Qin
DOI：
10.1099/ijs.0.02920-0
发表时间：
2004-05-01
影响因子：
2.8
作者：
de Boer, W;Leveau, JHJ;van Veen, JA
通讯作者：
van Veen, JA
影响因子：
3.3
作者：
Chernin, Leonid;Toklikishvili, Natela;Vainstein, Alexander
通讯作者：
Vainstein, Alexander
影响因子：
9.7
作者：
Baudoin, E;Benizri, E;Guckert, A
通讯作者：
Guckert, A