Temporal resolution in electrochemical imaging on single PC12 cells using amperometry and voltammetry at microelectrode arrays.
Temporal resolution in electrochemical imaging on single PC12 cells using amperometry and voltammetry at microelectrode arrays.
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DOI：
10.1021/ac102502g
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发表时间：
2011-01-15
影响因子：
7.4
通讯作者：
Ewing, Andrew G.
中科院分区：
文献类型：
--
作者：
Zhang, Bo;Heien, Michael L. A. V.;Santillo, Michael F.;Mellander, Lisa;Ewing, Andrew G.
碳纤维微电极阵列（MEA）已被用于电化学成像神经化学分泌从个别嗜铬细胞瘤（PC 12）细胞。多巴胺释放事件电化学监测从七个不同的位置上的单个PC 12细胞使用交替恒电位安培法和快速扫描循环伏安法（FSCV）。与安培法相比，循环伏安法可以提供优异的化学分辨率;然而，空间和时间分辨率都受到损害。这两种方法的空间和时间分辨率进行了定量比较，并解释使用模型的分子扩散在电极和细胞之间的纳米间隙的差异。分子流的数值模拟表明，多巴胺分子的扩散和电化学反应在电化学成像的时间分辨率都起着重要的作用。模拟还表明，扩散和电极电位的差异，在比较电流法和FSCV电极之间的信号串扰。
Carbon-fiber-microelectrode arrays (MEAs) have been utilized to electrochemically image neurochemical secretion from individual pheochromocytoma (PC12) cells. Dopamine release events were electrochemically monitored from seven different locations on single PC12 cells using alternately constant-potential amperometry and fast-scan cyclic voltammetry (FSCV). Cyclic voltammetry, when compared to amperometry, can provide excellent chemical resolution; however, spatial and temporal resolution are both compromised. The spatial and temporal resolution of these two methods has been quantitatively compared, and the differences explained using models of molecular diffusion at the nanogap between the electrode and the cell. A numerical simulation of the molecular flux reveals that the diffusion of dopamine molecules and electrochemical reactions both play important roles in the temporal resolution of electrochemical imaging. The simulation also reveals that the diffusion and electrode potential cause the differences in signal crosstalk between electrodes when comparing amperometry and FSCV.
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影响因子：
4.2
作者：
Heien, MLAV;Phillips, PEM;Wightman, RM
通讯作者：
Wightman, RM
影响因子：
5.3
作者：
Sombers, LA;Hanchar, HJ;Ewing, AG
通讯作者：
Ewing, AG
DOI：
10.1073/pnas.232702599
发表时间：
2003-01-21
影响因子：
11.1
作者：
Cans, AS;Wittenberg, N;Ewing, A
通讯作者：
Ewing, A
影响因子：
7.4
作者：
Zhang, Bo;Adams, Kelly L.;Ewing, Andrew G.
通讯作者：
Ewing, Andrew G.
影响因子：
3
作者：
Anderson, BB;Zerby, SE;Ewing, AG
通讯作者：
Ewing, AG