The Climate-Driven Genetic Diversity Has a Higher Impact on the Population Structure of Plasmopara viticola Than the Production System or QoI Fungicide Sensitivity in Subtropical Brazil.
The Climate-Driven Genetic Diversity Has a Higher Impact on the Population Structure of Plasmopara viticola Than the Production System or QoI Fungicide Sensitivity in Subtropical Brazil.
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DOI：
10.3389/fmicb.2020.575045
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发表时间：
2020
影响因子：
5.2
通讯作者：
Amorim L
中科院分区：
文献类型：
--
作者：
Santos RF;Ciampi-Guillardi M;Fraaije BA;de Oliveira AA;Amorim L
霜霉病，由葡萄生单轴霉引起，是影响巴西亚热带葡萄园的主要病害。在这里，我们收集了94 P.葡萄生菌株从四个有机和传统的葡萄园在两个主要的葡萄种植国家的巴西评估的敏感性醌外部抑制剂（QoI）嘧菌酯的表型和基因分型测定。利用10个微卫星标记研究了葡萄疫霉菌的地理分布、生产系统和对QoI杀菌剂的敏感性对种群遗传和结构的影响。细胞色素B序列分析表明，28和100%的葡萄园根据有机和常规管理进行G143 A突变，分别。G143A突变与高水平的嘧菌酯耐药性相关。分析的94个分离物中有3个携带先前在葡萄生疫霉中未描述的M125I改变，与野生型分离物相比，这与嘧菌酯敏感性降低5倍有关。基于细胞色素B基因序列的单倍型网络分析表明，巴西人口更密切相关的欧洲比北美人口。共鉴定出六种单倍型，其中两种携带G143A突变。微卫星分析显示，高等位基因和基因型变异的四个群体。种群分化分析表明，原产地直接影响葡萄疫霉的种群生物学特性，而生产系统和生活质量敏感性影响不大。在南里奥格兰德州观察到巨大的遗传多样性、有性繁殖和高水平的混合物。相比之下，在圣保罗州的人口占主导地位的几个克隆基因型，并没有混合基因型之间检测到的两个基因库中确定的状态。本研究提出了冬季气候条件影响葡萄霜霉病菌越冬生存策略的假设，并对葡萄霜霉病菌种群生物学产生了深远的影响。
Downy mildew, caused by Plasmopara viticola, is the main disease affecting vineyards in subtropical Brazil. Here, we collected 94 P. viticola isolates from four organic and conventional vineyards in the two main grape-growing states of Brazil to evaluate the sensitivity to the quinone outside inhibitor (QoI) azoxystrobin by pheno- and genotyping assays. The impact of location, production system and sensitivity to QoI fungicides on the population genetics and structure of P. viticola was determined using 10 microsatellite markers. Cytochrome b sequencing revealed that 28 and 100% of the isolates from vineyards under organic and conventional management carried the G143A mutation, respectively. The G143A mutation was associated with high levels of azoxystrobin resistance. Three out of the 94 isolates analyzed carried the M125I alteration, not previously described in P. viticola, which was associated with a five-fold reduction in azoxystrobin sensitivity compared to wild-type isolates. Haplotype network analysis based on cytochrome b gene sequences suggested that the Brazilian populations are more closely related to the European than the North American population. A total of six haplotypes were identified, with two of them carrying the G143A mutation. Microsatellite analysis revealed high allelic and genotypic variation among the four populations. Population differentiation analyses indicated that state of origin directly influences the population biology of P. viticola, while production system and QoI sensitivity have little effect. Great genetic diversity, sexual reproduction and high levels of admixture were observed in Rio Grande do Sul State. In contrast, populations in São Paulo State were dominated by a few clonal genotypes, and no admixed genotype was detected between the two genetic pools identified in the state. This study raises the hypothesis that winter weather conditions influence the overwinter survival strategy with profound effects in the population biology of P. viticola.
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DOI：
10.1111/j.1471-8286.2005.01240.x
发表时间：
2006-06-01
期刊：
MOLECULAR ECOLOGY NOTES
影响因子：
--
作者：
Delmotte, F.;Chen, W. J.;Corio-Costet, M. F.
通讯作者：
Corio-Costet, M. F.
影响因子：
4.1
作者：
Furuya, Seiichi;Mochizuki, Mai;Suzuki, Shunji
通讯作者：
Suzuki, Shunji
DOI：
10.1046/j.1471-8278.2000.00014.x
发表时间：
2001-03-01
期刊：
MOLECULAR ECOLOGY NOTES
影响因子：
--
作者：
Agapow, PM;Burt, A
通讯作者：
Burt, A
影响因子：
4.1
作者：
Fontaine, Severine;Remuson, Florent;Barres, Benoit
通讯作者：
Barres, Benoit
影响因子：
7.7
作者：
Excoffier, Laurent;Lischer, Heidi E. L.
通讯作者：
Lischer, Heidi E. L.