Selective expression of the immediate early gene c-jun in axotomized rat medial septal neurons is not related to neuronal degeneration
Selective expression of the immediate early gene c-jun in axotomized rat medial septal neurons is not related to neuronal degeneration
复制标题
轴突大鼠内侧间隔神经元中立即早期基因 c-jun 的选择性表达与神经元变性无关
作者：
C. Haas;T. Deller;T. Naumann;M. Frotscher
在本研究中，我们使用解剖学上定义良好的隔海马投射来研究神经元对轴突切断反应的分子事件。本实验观察了大鼠穹窿海马伞切断后隔海马神经元中3种即早基因（c-fos、c-jun和jun B）的表达。此外，通过分析DNA片段化来评估隔复合体的逆行变性程度。在术后时间过程分析中，c-jun免疫反应性（IR）在内侧隔/斜角带复合体（MSDB）的神经元的核中观察到强烈的增加postaxotomy后2和6 d，随后分别在12 d和3周后下降。FFT后9周，c-jun IR消失。c-jun阳性的MS神经元被确定为前隔海马投射细胞的双重标记与逆行运输示踪剂Fluoro-Gold注射到海马轴突切断前。在与免疫细胞化学数据，有一个大规模的诱导c-jun mRNA的轴突切断MS神经元可视化原位杂交组织化学。未手术或损伤的内侧隔神经元均未检测到c-fos mRNA和c-fos或jun B IR。实验使用TdT介导的脱氧尿苷三磷酸缺口末端标记技术，旨在检测核DNA片段变性神经元，补充了这项研究。在研究的术后时间范围内，MS神经元没有表现出DNA片段化。我们的结论是MSDB神经元存活轴突切断FFT和显示特征性的基因表达的变化。
In the present study, we use the anatomically well defined septohippocampal projection to study the molecular events involved in the reaction of neurons to axotomy. The expression of three immediate early genes (c-fos, c-jun, and jun B) was investigated in rat septohippocampal neurons after axotomy by bilateral fimbria-fornix transection (FFT). Moreover, the extent of retrograde degeneration in the septal complex was assessed by analyzing DNA fragmentation. In a postoperative time course analysis, a strong increase of c-jun immunoreactivity (IR) was observed in the nuclei of neurons in the medial septum/diagonal band complex (MSDB) 2 and 6 d postaxotomy, which was followed by a decline after 12 d and 3 weeks, respectively. Nine weeks after FFT, c-jun IR had disappeared. The c-jun-positive MS neurons were identified as former septohippocampal projection cells by double-labeling with the retrogradely transported tracer Fluoro-Gold injected into the hippocampus before axotomy. In line with the immunocytochemical data, there was a massive induction of c-jun mRNA in the axotomized MS neurons as visualized by in situ hybridization histochemistry. c-fos mRNA and c-fos or jun B IR were not detectable in either unoperated or lesioned medial septal neurons. Experiments using the TdT-mediated deoxyuridine triphosphate nick-end-labeling technique, designed to detect nuclear DNA fragmentation in degenerating neurons, complemented this study. During the postoperative time range studied, MS neurons did not exhibit DNA fragmentation. We conclude that MSDB neurons survive axotomy by FFT and display characteristic changes in gene expression.
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受损胆碱能神经元的存活、生长和功能。
DOI：
10.1007/978-3-0348-9138-7_26
发表时间：
1989
期刊：
EXS
影响因子：
--
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DOI：
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发表时间：
1986-12-01
影响因子：
11.1
作者：
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通讯作者：
GAGE, FH
影响因子：
10.5
作者：
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通讯作者：
CURRAN, T
影响因子：
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作者：
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DOI：
10.1073/pnas.89.10.4387
发表时间：
1992
影响因子：
11.1
作者：
Hahn,M;Hahn,SL;Stone,DM;Joh,TH
通讯作者：
Joh,TH