Micro-encapsulated essential oils and organic acids combination improves intestinal barrier function, inflammatory responses and microbiota of weaned piglets challenged with enterotoxigenic Escherichia coli F4 (K88(+)).
Micro-encapsulated essential oils and organic acids combination improves intestinal barrier function, inflammatory responses and microbiota of weaned piglets challenged with enterotoxigenic Escherichia coli F4 (K88(+)).
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DOI：
10.1016/j.aninu.2020.04.004
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发表时间：
2020-09
期刊：
影响因子：
--
通讯作者：
Piao X
中科院分区：
文献类型：
--
作者：
Xu Y;Lahaye L;He Z;Zhang J;Yang C;Piao X
本研究评价了微囊化（受保护）有机酸（OA）和精油（EO）组合（P（OA + EO））以及游离酸（FA）常规混合物对产肠毒素大肠杆菌（ETEC）F4（K88+）攻毒断奶仔猪的生长、免疫应答、肠道屏障和微生物群的影响。共30个杂交品种（杜×长×大白色）断奶公猪（7.41 ± 0.06 kg，28日龄）随机分为5组：1）未激发阳性对照（PC），2）ETEC F4（K88+）激发阴性对照（NC），3）NC +50 mg/kg吉他霉素+100 mg/kg喹乙醇+5 g/kg游离酸化剂（FA），4）NC +吉他霉素50 mg/kg +喹乙醇100 mg/kg + P（OA + EO）1 g/kg（P1）; 5）NC +吉他霉素50 mg/kg +喹乙醇100 mg/kg + P（OA + EO）2 g/kg（P2）。每个日粮处理设6个重复，每个重复1头仔猪，试验持续3周。第7天，NC、FA、P1和P2组猪经口给予10 mL ETEC F4（K88+）培养物（1 × 109 CFU/mL）。在ETEC F4（K88+）攻毒后第7 ~ 14天，与NC组和FA组相比，P1组显著提高了增料比（G：F）（P < 0.05）。从第14 ~ 21天，与NC组和FA组相比，P2组猪的平均日增重显著提高（P < 0.05）。与NC相比，P2降低ETEC F4（K88+）攻击后4 h收集的血清中肿瘤坏死因子-α（TNF-α）、白细胞介素（IL）-6和IL-10的浓度（P < 0.05）。与NC组相比，P1组回肠闭合蛋白和闭合小带-1蛋白表达增加（P < 0.05）。3周实验后，与PC相比，P2降低了肠道菌群的α多样性，P1增加了回肠、盲肠和结肠中乳酸杆菌的相对丰度（P < 0.05）。综上所述，在F4（K88+）攻毒后，2g/kg的P（OA + EO）添加剂与抗生素组合可以提高仔猪生产性能并减轻炎症，并且1g/kg的P（OA + EO）添加剂与抗生素组合可以改善肠道屏障并增加有益的微生物群组成。
This study evaluated the effects of micro-encapsulated (protected) organic acids (OA) and essential oils (EO) combination, P(OA + EO), and effects of a regular blend of free acids (FA) on the growth, immune responses, intestinal barrier and microbiota of weaned piglets challenged with enterotoxigenic Escherichia coli (ETEC) F4 (K88+). A total of 30 crossbred (Duroc × Landrace × Large White) weaned barrows (7.41 ± 0.06 kg, 28 d old) were assigned randomly to 5 treatments: 1) non-challenged positive control (PC), 2) ETEC F4 (K88+)-challenged negative control (NC), 3) NC + kitasamycin at 50 mg/kg + olaquindox at 100 mg/kg + free acidifier (FA) at 5 g/kg, 4) NC + kitasamycin at 50 mg/kg + olaquindox at 100 mg/kg + P(OA + EO) at 1 g/kg (P1), 5) NC + kitasamycin at 50 mg/kg + olaquindox at 100 mg/kg + P(OA + EO) at 2 g/kg (P2). Each dietary treatment had 6 replicates of one piglet each and the study lasted for 3 wk. On d 7, pigs in NC, FA, P1 and P2 were orally dosed with 10 mL of ETEC F4 (K88+) culture (1 × 109 CFU/mL). From d 7 to 14 after the ETEC F4 (K88+) challenge, P1 increased gain-to-feed ratio (G:F) significantly (P < 0.05) compared with NC and FA groups. From d 14 to 21, P2 increased the average daily gain of pigs (P < 0.05) compared with NC and FA groups. Compared with NC, P2 reduced tumor necrosis factor-α (TNF-α), interleukin (IL)-6 and IL-10 concentrations (P < 0.05) in sera collected at 4 h later after ETEC F4 (K88+) challenge. On d 21, P1 increased occludin and zonula occludens-1 protein expression in ileum compared with NC (P < 0.05). After this 3-wk experiment, alpha diversity of gut microbiota was decreased by P2 compared with PC, and P1 increased the relative abundance of Lactobacillus in ileum, cecum and colon (P < 0.05). In conclusion, dietary P(OA + EO) additive at 2 g/kg combined with antibiotics could improve piglet performance and attenuate inflammation, and P(OA + EO) additive at 1 g/kg combined with antibiotics improved intestinal barrier and increased beneficial microbiota composition after an F4 (K88+) challenge.
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影响因子：
5.2
作者：
Ilinskaya ON;Ulyanova VV;Yarullina DR;Gataullin IG
通讯作者：
Gataullin IG
影响因子：
3.4
作者：
Capaldo, Christopher T.;Nusrat, Asma
通讯作者：
Nusrat, Asma
影响因子：
4.4
作者：
Daudelin JF;Lessard M;Beaudoin F;Nadeau E;Bissonnette N;Boutin Y;Brousseau JP;Lauzon K;Fairbrother JM
通讯作者：
Fairbrother JM
影响因子：
3.4
作者：
Guttman, JA;Li, YL;Finlay, BB
通讯作者：
Finlay, BB
影响因子：
2.1
作者：
Fossum, C
通讯作者：
Fossum, C