Desulfovibrio vulgaris, a potent acetic acid-producing bacterium, attenuates nonalcoholic fatty liver disease in mice.
Desulfovibrio vulgaris, a potent acetic acid-producing bacterium, attenuates nonalcoholic fatty liver disease in mice.
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普通脱硫弧菌（Desulfovibrio vulgaris）是一种有效的产乙酸细菌，可减轻小鼠非酒精性脂肪肝病
DOI：
10.1080/19490976.2021.1930874
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发表时间：
2021-01
期刊：
影响因子：
12.2
通讯作者：
Li H
中科院分区：
文献类型：
--
作者：
Hong Y;Sheng L;Zhong J;Tao X;Zhu W;Ma J;Yan J;Zhao A;Zheng X;Wu G;Li B;Han B;Ding K;Zheng N;Jia W;Li H
新出现的证据支持使用益生元如草药衍生多糖通过调节肠道微生物群来治疗非酒精性脂肪性肝病（NAFLD）。本研究旨在研究蒙古黄芪多糖（Astragalus polysaccharides， APS）对高脂饲料（HFD）小鼠的微生物依赖性抗nafld作用。然而，APS对NAFLD形成有益作用的确切机制仍然知之甚少。采用共箱试验评价黄芪多糖的抗nafld作用。然后，采用靶向代谢组学和宏基因组学对APS特异性富集的短链脂肪酸（SCFAs）和细菌进行检测。进一步进行体外实验，测试鉴定出的细菌产生scfas的能力。最后，在饲喂hfd的小鼠中检测鉴定出的细菌抗nafld的效果。我们的研究结果首次证明了黄芪多糖对hfd喂养小鼠的抗nafld作用和肠道微生物群的贡献。此外，我们的研究结果表明，在体外实验和补充aps的小鼠中，SCFAs（主要是乙酸）升高。宏基因组学研究表明，Desulfovibrio属的D. vulgaris不仅富含APS，而且是一种强有力的乙酸产生物，对食源性食油喂养的小鼠具有显著的抗nafld作用。此外，寻常草还能调节肝脏脂质代谢基因表达模式，特别是抑制肝脏脂肪酸合成酶（FASN）和CD36蛋白的表达。本研究结果表明，黄芪多糖可能通过产生乙酸和调节小鼠肝脏脂质代谢来减轻肝脏脂肪变性。我们还需要进一步的研究来探究寻常草对宿主代谢的长期影响及其机制。
The emerging evidence supports the use of prebiotics like herb-derived polysaccharides for treating nonalcoholic fatty liver disease (NAFLD) by modulating gut microbiome. The present study was initiated on the microbiota-dependent anti-NAFLD effect of Astragalus polysaccharides (APS) extracted from Astragalus mongholicus Bunge in high-fat diet (HFD)-fed mice. However, the exact mechanisms underlying the beneficial effects of APS on NAFLD formation remain poorly understood. Co-housing experiment was used to assess the microbiota dependent anti-NAFLD effect of APS. Then, targeted metabolomics and metagenomics were adopted for determining short-chain fatty acids (SCFAs) and bacteria that were specifically enriched by APS. Further in vitro experiment was carried out to test the capacity of SCFAs-producing of identified bacterium. Finally, the anti-NAFLD efficacy of identified bacterium was tested in HFD-fed mice. Our results first demonstrated the anti-NAFLD effect of APS in HFD-fed mice and the contribution of gut microbiota. Moreover, our results indicated that SCFAs, predominantly acetic acid were elevated in APS-supplemented mice and ex vivo experiment. Metagenomics revealed that D. vulgaris from Desulfovibrio genus was not only enriched by APS, but also a potent generator of acetic acid, which showed significant anti-NAFLD effects in HFD-fed mice. In addition, D. vulgaris modulated the hepatic gene expression pattern of lipids metabolism, particularly suppressed hepatic fatty acid synthase (FASN) and CD36 protein expression. Our results demonstrate that APS enriched D. vulgaris is effective on attenuating hepatic steatosis possibly through producing acetic acid, and modulation on hepatic lipids metabolism in mice. Further studies are warranted to explore the long-term impacts of D. vulgaris on host metabolism and the underlying mechanism.
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影响因子：
5.2
作者：
Burrichter, Anna;Dengerl, Karin;Schleheck, David
通讯作者：
Schleheck, David
影响因子：
12.2
作者：
Holscher HD
通讯作者：
Holscher HD
DOI：
10.1002/path.4659
发表时间：
2016-01
期刊：
The Journal of pathology
影响因子：
--
作者：
Carter RN;Morton NM
通讯作者：
Morton NM
影响因子：
12.2
作者：
Du J;Zhang P;Luo J;Shen L;Zhang S;Gu H;He J;Wang L;Zhao X;Gan M;Yang L;Niu L;Zhao Y;Tang Q;Tang G;Jiang D;Jiang Y;Li M;Jiang A;Jin L;Ma J;Shuai S;Bai L;Wang J;Zeng B;Wu D;Li X;Zhu L
通讯作者：
Zhu L
影响因子：
5.6
作者：
Huang YC;Tsay HJ;Lu MK;Lin CH;Yeh CW;Liu HK;Shiao YJ
通讯作者：
Shiao YJ