In situ measurement of transport between subchondral bone and articular cartilage.
In situ measurement of transport between subchondral bone and articular cartilage.
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DOI：
10.1002/jor.20883
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发表时间：
2009-10
影响因子：
2.8
通讯作者：
Wang, Liyun
中科院分区：
文献类型：
--
作者：
Pan, Jun;Zhou, Xiaozhou;Li, Wen;Novotny, John E.;Doty, Stephen B.;Wang, Liyun
软骨下骨和关节软骨在关节的承载中起着互补的作用。尽管软骨下骨和关节软骨之间的生物力学耦合已经建立，但它们之间是否存在直接的生物化学联系仍不清楚。之前，人们普遍认为这两个隔室之间的钙化软骨不能渗透到溶质和气体的运输中。然而，最近的研究发现，小分子可以从软骨下骨渗透到钙化的软骨中。为了对钙化软骨中溶质的实时传输进行定量，我们开发了一种基于光漂白(FLIP)引起的荧光损失的成像方法。荧光素钠(376Da)在小鼠股骨远端软骨下与钙化软骨之间和钙化软骨内的扩散系数分别为0.07±0.03和0.26±0.22μm2/s。电子显微镜观察发现，钙化软骨基质中含有非矿化区(∼体积分数为22%)，这些非矿化区或者是矿层之间的大片(53±18 nm)，或者是矿层内大量的小区域(4.5±0.8 nm)，可能是矿物质的运输途径。这些结果提示，软骨下骨和关节软骨之间可能存在直接的信号传递，它们形成了一个既有机械作用又有生化作用的功能单位，可能在关节的维持和退变中发挥作用。
Subchondral bone and articular cartilage play complementary roles in load bearing of the joints. Although the biomechanical coupling between subchondral bone and articular cartilage is well established, it remains unclear whether direct biochemical communication exists between them. The calcified cartilage between these two compartments was generally believed to be impermeable to transport of solutes and gases, previously. However, recent studies found that small molecules could penetrate into the calcified cartilage from the subchondral bone. To quantify the real-time solute transport across the calcified cartilage, we developed a novel imaging method based on fluorescence loss induced by photobleaching (FLIP). Diffusivity of sodium fluorescein (376 Da) was quantified to be 0.07±0.03 and 0.26±0.22 μm2/sec between subchondral bone and calcified cartilage and within the calcified cartilage in the murine distal femur, respectively. Electron microscopy revealed that calcified cartilage matrix contained non-mineralized regions (∼22% volume fraction) that are either large patches (53±18 nm) among the mineral deposits or numerous small regions (4.5±0.8 nm) within the mineral deposits, which may serve as transport pathways. These results suggest that there exists a possible direct signaling between subchondral bone and articular cartilage, and they form a functional unit with both mechanical and biochemical interactions, which may play a role in the maintenance and degeneration of the joint.
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影响因子：
--
作者：
Hwang, Jennifer;Bae, Won C.;Shieu, Wendy;Lewis, Chad W.;Bugbee, William D.;Sah, Robert L.
通讯作者：
Sah, Robert L.
影响因子：
--
作者：
Westacott, CI;Webb, GR;Elson, CJ
通讯作者：
Elson, CJ
DOI：
10.1073/pnas.0505193102
发表时间：
2005-08-16
影响因子：
11.1
作者：
Wang, LY;Wang, YL;Schaffler, MB
通讯作者：
Schaffler, MB
影响因子：
5
作者：
Martel-Pelletier, Johanne;Lajeunesse, Daniel;Pelletier, Jean-Pierre
通讯作者：
Pelletier, Jean-Pierre
DOI：
10.1016/j.bbrc.2005.10.025
发表时间：
2005-12-16
影响因子：
3.1
作者：
Jiang, J;Nicoll, SB;Lu, HH
通讯作者：
Lu, HH