Empirically controlled mapping of the Caenorhabditis elegans protein-protein interactome network.
Empirically controlled mapping of the Caenorhabditis elegans protein-protein interactome network.
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DOI：
10.1038/nmeth.1279
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发表时间：
2009-01
期刊：
影响因子：
48
通讯作者：
Vidal, Marc
中科院分区：
文献类型：
--
作者：
Simonis, Nicolas;Rual, Jean-Francois;Carvunis, Anne-Ruxandra;Tasan, Murat;Lemmens, Irma;Hirozane-Kishikawa, Tomoko;Hao, Tong;Sahalie, Julie M.;Venkatesan, Kavitha;Gebreab, Fana;Cevik, Sebiha;Klitgord, Niels;Fan, Changyu;Braun, Pascal;Li, Ning;Ayivi-Guedehoussou, Nono;Dann, Elizabeth;Bertin, Nicolas;Szeto, David;Dricot, Ameli;Yildirim, Muhammed A.;Lin, Chenwei;de Smet, Anne-Sophie;Kao, Huey-Ling;Simon, Christophe;Smolyar, Alex;Ahn, Jin Sook;Tewari, Muneesh;Boxem, Mike;Milstein, Stuart;Yu, Haiyuan;Dreze, Matija;Vandenhaute, Jean;Gunsalus, Kristin C.;Cusick, Michael E.;Hill, David E.;Tavernier, Jan;Roth, Frederick P.;Vidal, Marc
为了提供准确的生物学假设并了解细胞网络的全局特性，蛋白质-蛋白质相互作用的系统鉴定必须满足高质量的标准。我们提出了一个扩展的秀丽隐杆线虫蛋白质-蛋白质相互作用网络，或“相互作用组”图，该图来源于使用高特异性高通量酵母双杂交系统测试约10,000 ×约10,000个蛋白质的矩阵。通过一个新的质量控制经验框架，我们证明了所得到的数据集（Worm Interactome 2007或WI-2007）在质量上与从文献中整理的低通量数据相似。以前的交互数据集已被过滤并与WI-2007集成，以生成高可信度的综合地图（Worm Interactome version 8或WI8）。这项工作使我们能够估计蠕虫相互作用组的大小约为116,000个相互作用。与其他类型的功能基因组数据的比较表明，不同的实验方法在预测基因或蛋白质之间不同的功能关系特征方面具有互补性。
To provide accurate biological hypotheses and inform upon global properties of cellular networks, systematic identification of protein–protein interactions has to meet high-quality standards. We present an expanded Caenorhabditis elegans protein-protein interaction network, or “interactome” map derived from testing a matrix of ~ 10,000 × ~ 10,000 proteins using a highly specific high-throughput yeast two-hybrid system. Through a new quality control empirical framework, We show that the resulting dataset (Worm Interactome 2007 or WI-2007) is similar in quality to low-throughput data curated from the literature. Previous interaction datasets have been filtered and integrated with WI-2007 to generate a high confidence consolidated map (Worm Interactome version 8 or WI8). This work allows us to estimate the size of the worm interactome at ~ 116,000 interactions. Comparison with other types of functional genomic data shows the complementarity of distinct experimental approaches in predicting different functional relationship features between genes or proteins.
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影响因子：
56.9
作者：
Walhout, AJM;Sordella, R;Vidal, M
通讯作者：
Vidal, M
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56.9
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48
作者：
Venkatesan, Kavitha;Rual, Jean-Francois;Vazquez, Alexei;Stelzl, Ulrich;Lemmens, Irma;Hirozane-Kishikawa, Tomoko;Hao, Tong;Zenkner, Martina;Xin, Xiaofeng;Goh, Kwang-Il;Yildirim, Muhammed A.;Simonis, Nicolas;Heinzmann, Kathrin;Gebreab, Fana;Sahalie, Julie M.;Cevik, Sebiha;Simon, Christophe;de Smet, Anne-Sophie;Dann, Elizabeth;Smolyar, Alex;Vinayagam, Arunachalam;Yu, Haiyuan;Szeto, David;Borick, Heather;Dricot, Amelie;Klitgord, Niels;Murray, Ryan R.;Lin, Chenwei;Lalowski, Maciej;Timm, Jan;Rau, Kirstin;Boone, Charles;Braun, Pascal;Cusick, Michael E.;Roth, Frederick P.;Hill, David E.;Tavernier, Jan;Wanker, Erich E.;Barabasi, Albert-Laszlo;Vidal, Marc
通讯作者：
Vidal, Marc