Cellular and subcellular localization of estrogen and progestin receptor immunoreactivities in the mouse hippocampus.
Cellular and subcellular localization of estrogen and progestin receptor immunoreactivities in the mouse hippocampus.
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DOI：
10.1002/cne.22361
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发表时间：
2010-07-15
期刊：
影响因子：
--
通讯作者：
Milner TA
中科院分区：
文献类型：
--
作者：
Mitterling KL;Spencer JL;Dziedzic N;Shenoy S;McCarthy K;Waters EM;McEwen BS;Milner TA
雌激素受体-α（ERα）、-β（ERβ）和孕激素受体（PR）在大鼠海马结构的分布主要在胞核内。由于大鼠和小鼠在细胞水平上对雌二醇治疗的反应不同，本研究检测了小鼠背海马结构中卵巢激素受体的分布。为此，通过光和电子免疫显微镜在整个发情周期的雄性和雌性小鼠中定位ERα、ERβ和PR抗体。小鼠海马结构的光镜检查显示，稀疏的细胞核ERα-和PR-免疫反应（-ir）在CA 1区最突出，弥漫性ERβ-ir主要在CA 1锥体细胞层以及少数中间神经元中。超微结构分析还显示，在整个海马结构的神经元和神经胶质细胞中，ERα-、ERβ-和PR-ir呈离散分布。虽然在所有检查的动物组中均检测到了细胞核轮廓，但轮廓的数量和类型因性别和发情周期阶段而异。ERα-ir在动情间期雌鼠中最高，尤其在树突棘、轴突和胶质细胞中。雌鼠动情期和间情期ERβ-ir表达最高，主要分布于树突棘和胶质细胞。相反，PR-IR是最高的发情前期，主要是在轴突。除了小鼠苔藓纤维末端中非常低水平的胞核ERβ-ir外，所有三种抗体在小鼠中的标记模式与先前在大鼠中发现的超微结构标记相似，表明这些受体的调节在两个物种中是保守的。
Estrogen receptor-α (ERα), -β (ERβ) and progestin receptor (PR) immunoreactivities are localized to extranuclear sites in the rat hippocampal formation. Since rats and mice respond differently to estradiol treatment at a cellular level, the present study examined the distribution of ovarian hormone receptors in the dorsal hippocampal formation of mice. For this, antibodies to ERα, ERβ, and PR were localized by light and electron immunomicroscopy in male and female mice across the estrous cycle. Light microscopic examination of the mouse hippocampal formation showed sparse nuclear ERα–, and PR-immunoreactivity (-ir) most prominent in the CA1 region and diffuse ERβ-ir primarily in the CA1 pyramidal cell layer as well as in a few interneurons. Ultrastructural analysis additionally revealed discrete extranuclear ERα-, ERβ- and PR-ir in neuronal and glial profiles throughout the hippocampal formation. While extranuclear profiles were detected in all animal groups examined, the amount and types of profiles varied with sex and estrous cycle phase. ERα-ir was highest in diestrus females, particularly in dendritic spines, axons and glia. Similarly, ERβ-ir was highest in estrus and diestrus females, mainly in dendritic spines and glia. Conversely, PR-ir was highest during proestrus, and mostly in axons. Except for very low levels of extranuclear ERβ-ir in mossy fiber terminals in mice, the labeling patterns in the mice for all three antibodies were similar to the ultrastructural labeling found previously in rats, suggesting that regulation of these receptors is well conserved across the two species.
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