Arabidopsis ABA receptor RCAR1/PYL9 interacts with an R2R3-type MYB transcription factor, AtMYB44.
Arabidopsis ABA receptor RCAR1/PYL9 interacts with an R2R3-type MYB transcription factor, AtMYB44.
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拟南芥 ABA 受体 RCAR1/PYL9 与 R2R3 型 MYB 转录因子 AtMYB44 相互作用
DOI：
10.3390/ijms15058473
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发表时间：
2014-05-13
影响因子：
5.6
通讯作者：
Yang Y
中科院分区：
文献类型：
--
作者：
Li D;Li Y;Zhang L;Wang X;Zhao Z;Tao Z;Wang J;Wang J;Lin M;Li X;Yang Y
脱落酸（阿坝）信号在植物生长发育和逆境适应中起着重要作用。RCAR 1/PYL 9是拟南芥细胞质和细胞核的阿坝受体。为了进一步了解RCAR 1/PYL 9的调控机制，进行酵母双杂交方法以筛选RCAR 1/PYL 9相互作用蛋白，并鉴定了R2 R3型MYB转录因子AtMYB 44。通过谷胱甘肽S-转移酶（GST）下拉和双分子荧光互补（BiFC）测定进一步证实了RCAR 1/PYL 9和AtMYB 44之间的相互作用。基因表达分析表明，AtMYB 44负调控ABA反应基因RAB 18的表达，与RCAR 1/PYL 9报道的相反的作用。竞争性GST pull-down分析和磷酸酶活性分析表明，AtMYB 44和ABI 1竞争结合RCAR 1/PYL 9，从而降低了阿坝存在下RCAR 1/PYL 9对ABI 1磷酸酶活性的抑制作用。此外，在原生质体中的瞬时激活试验显示AtMYB 44可能也降低了RCAR 1/PYL 9介导的ABI 1活性抑制。综上所述，我们的工作为AtMYB 44参与阿坝信号转导提供了一个合理的分子机制。
Abscisic acid (ABA) signaling plays important roles in plant growth, development and adaptation to various stresses. RCAR1/PYL9 has been known as a cytoplasm and nuclear ABA receptor in Arabidopsis. To obtain further insight into the regulatory mechanism of RCAR1/PYL9, a yeast two-hybrid approach was performed to screen for RCAR1/PYL9-interacting proteins and an R2R3-type MYB transcription factor, AtMYB44, was identified. The interaction between RCAR1/PYL9 and AtMYB44 was further confirmed by glutathione S-transferase (GST) pull-down and bimolecular fluorescence complementation (BiFC) assays. Gene expression analysis showed that AtMYB44 negatively regulated the expression of ABA-responsive gene RAB18, in contrast to the opposite role reported for RCAR1/PYL9. Competitive GST pull-down assay and analysis of phosphatase activity demonstrated that AtMYB44 and ABI1 competed for binding to RCAR1/PYL9 and thereby reduced the inhibitory effect of RCAR1/PYL9 on ABI1 phosphatase activity in the presence of ABA in vitro. Furthermore, transient activation assay in protoplasts revealed AtMYB44 probably also decreased RCAR1/PYL9-mediated inhibition of ABI1 activity in vivo. Taken together, our work provides a reasonable molecular mechanism of AtMYB44 in ABA signaling.
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DOI：
10.1126/science.1173041
发表时间：
2009-05-22
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Park SY;Fung P;Nishimura N;Jensen DR;Fujii H;Zhao Y;Lumba S;Santiago J;Rodrigues A;Chow TF;Alfred SE;Bonetta D;Finkelstein R;Provart NJ;Desveaux D;Rodriguez PL;McCourt P;Zhu JK;Schroeder JI;Volkman BF;Cutler SR
通讯作者：
Cutler SR
影响因子：
5.1
作者：
LANG, V;PALVA, ET
通讯作者：
PALVA, ET
DOI：
10.1111/j.1365-313x.2009.04054.x
发表时间：
2010-01
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Nishimura N;Sarkeshik A;Nito K;Park SY;Wang A;Carvalho PC;Lee S;Caddell DF;Cutler SR;Chory J;Yates JR;Schroeder JI
通讯作者：
Schroeder JI
影响因子：
6.4
作者：
MURASHIGE, T;SKOOG, F
通讯作者：
SKOOG, F
影响因子：
9.4
作者：
Licausi, Francesco;Weits, Daan A.;van Dongen, Joost T.
通讯作者：
van Dongen, Joost T.