A Scalable, Modular Degasser for Passive In-Line Removal of Bubbles from Biomicrofluidic Devices.
A Scalable, Modular Degasser for Passive In-Line Removal of Bubbles from Biomicrofluidic Devices.
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DOI：
10.3390/mi14020435
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发表时间：
2023-02-11
期刊：
影响因子：
3.4
通讯作者：
Pompano, Rebecca R.
中科院分区：
文献类型：
--
作者：
Musgrove, Hannah B.;Saleheen, Amirus;Zatorski, Jonathan M.;Arneja, Abhinav;Luckey, Chance John;Pompano, Rebecca R.
气泡是微流体故障的常见原因，因为它们会扰乱设备的微尺寸特征内的流体流动。由于气泡很容易在温热细胞培养试剂中形成，因此生物微流控系统通常需要脱气。然而，可以与现有芯片模块化使用的微型脱气器的制造可能会很麻烦或具有挑战性，特别是对于没有配备传统微制造设备的实验室，而且目前的商业选择可能很昂贵。在这里，我们解决了对一种负担得起的、可获得的气泡捕集器的需求，该捕气器可以在线用于芯片上器官和其他微流控培养的持续灌流。我们对之前描述的手工制造的PDMS脱气器进行了改装，使其能够通过商业机械加工或熔融沉积成型(FDM)3D打印进行放大、可重复制造。经过优化，机械加工和3D打印的脱气器在恒定灌流下稳定2周，没有泄漏。在L室体积约140ug的情况下，根据气泡形成的速度，外推捕集容量以允许~5-20周的脱气。这些除气器与细胞培养是生物相容的，它们成功地阻止了气泡到达下游的微流控设备。两种除气器材料都没有或很少发生淋洗。机加工脱气器不吸收试剂，而FDM型印刷脱气器只吸收少量试剂，在压力驱动流量下，两者都保持了1微米L/分钟至1毫升/分钟的流体完整性。因此，这些除气器可以批量制造，并允许在简单的微流控灌流装置中长期、有效地去除气泡。
Bubbles are a common cause of microfluidic malfunction, as they can perturb the fluid flow within the micro-sized features of a device. Since gas bubbles form easily within warm cell culture reagents, degassing is often necessary for biomicrofluidic systems. However, fabrication of a microscale degasser that can be used modularly with pre-existing chips may be cumbersome or challenging, especially for labs not equipped for traditional microfabrication, and current commercial options can be expensive. Here, we address the need for an affordable, accessible bubble trap that can be used in-line for continuous perfusion of organs-on-chip and other microfluidic cultures. We converted a previously described, manually fabricated PDMS degasser to allow scaled up, reproducible manufacturing by commercial machining or fused deposition modeling (FDM) 3D printing. After optimization, the machined and 3D printed degassers were found to be stable for >2 weeks under constant perfusion, without leaks. With a ~140 µL chamber volume, trapping capacity was extrapolated to allow for ~5–20 weeks of degassing depending on the rate of bubble formation. The degassers were biocompatible for use with cell culture, and they successfully prevented bubbles from reaching a downstream microfluidic device. Both degasser materials showed little to no leaching. The machined degasser did not absorb reagents, while the FDM printed degasser absorbed a small amount, and both maintained fluidic integrity from 1 µL/min to >1 mL/min of pressure-driven flow. Thus, these degassers can be fabricated in bulk and allow for long-term, efficient bubble removal in a simple microfluidic perfusion set-up.
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影响因子：
2.4
作者：
Knight E;Przyborski S
通讯作者：
Przyborski S
DOI：
10.1016/j.ooc.2022.100018
发表时间：
2022-12-01
期刊：
Organs-on-a-chip
影响因子：
--
作者：
Ortiz-Cardenas, Jennifer E;Zatorski, Jonathan M;Pompano, Rebecca R
通讯作者：
Pompano, Rebecca R
影响因子：
3.2
作者：
Wang, Limu;Zhang, Mengying;Wen, Weijia
通讯作者：
Wen, Weijia
影响因子：
3.4
作者：
Williams, Matthew J.;Lee, Nicholas K.;Abhyankar, Vinay V.
通讯作者：
Abhyankar, Vinay V.
影响因子：
4.6
作者：
Yang, Z;Matsumoto, S;Maeda, R
通讯作者：
Maeda, R