Enhanced culture method for detection of replication-competent virus in peripheral blood mononuclear cells of HIV type 1-infected individuals.
Enhanced culture method for detection of replication-competent virus in peripheral blood mononuclear cells of HIV type 1-infected individuals.
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用于检测 HIV 1 型感染者外周血单核细胞中具有复制能力的病毒的增强培养方法。
DOI：
10.1089/088922202753747923
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Somasundaran,Mohan
中科院分区：
文献类型：
--
作者：
Haran,JohnP;Greenough,ThomasC;Luzuriaga,Katherine;Sullivan,JohnL;Flanigan,Timothy;Somasundaran,Mohan
在感染个体中检测具有复制能力的人类免疫缺陷病毒1型（HIV-1）是研究病毒潜伏期和治疗期间持续复制库的必要条件。我们描述了一种改进的共培养方法，用于检测/分离血浆病毒载量低或检测不到的hiv -1感染个体的病毒。我们观察到hiv -1未感染供者CD4+T细胞上CD4和趋化因子受体浓度有很大的变化。我们从有丝分裂原刺激后表达高水平CCR5的供体中选择细胞，与hiv -1感染者外周血单个核细胞结合培养。使用这种供体增强培养方法，从长期非进展性感染的无症状成人和接受有效的高活性抗逆转录病毒治疗的儿童中分离出病毒，而与表达较低水平CCR5的细胞平行培养则没有。从先前使用报告的方法进行的所有尝试均未成功的个体中分离出病毒。在多个采样时间点检测到来自同一个体的冷冻保存细胞的病毒生长，包括测量病毒基因组2-LTR（长末端重复）环状形式的敏感试验为阴性的时间点。这将是研究成人和儿童人群中病毒潜伏期和HIV-1发病机制的有用技术。
The detection of replication-competent human immunodeficiency virus type 1 (HIV-1) in infected individuals is integral for studies of viral latency and reservoirs of continuing replication during treatment. We describe a modified coculture method to detect/isolate virus from HIV-1-infected individuals with low or undetectable plasma viral loads. We observed a wide range in CD4 and chemokine receptor concentrations on CD4+T cells of HIV-1-uninfected donors. We selected cells from donors who expressed high levels of CCR5 after mitogen stimulation to combine in culture with peripheral blood mononuclear cells of HIV-1-infected individuals. Using this donor-enhanced culture method, viruses were isolated from asymptomatic adults with longterm nonprogressive infection, and children receiving effective highly active antiretroviral therapy, whereas parallel cultures with cells expressing lower levels of CCR5 yielded none. Virus was isolated from an individual for whom all previous attempts using reported methods were unsuccessful. Virus growth from cryopreserved cells from this same individual was detected at multiple sampling time points, including a time point at which a sensitive assay to measure 2-LTR (long terminal repeat) circular forms of the viral genome was negative. This will be a useful technique by which to study viral latency and HIV-1 pathogenesis in adult and pediatric populations.
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单 LTR HIV-1 DNA 连接数量的增加与体内 HIV-1 抗原表达和 CD4+ 细胞下降相关
影响因子：
12.7
作者：
S. Jurriaans;J. Dekker;M. Cornelissen;J. Goudsmit;A. De ronde
通讯作者：
A. De ronde
影响因子：
64.8
作者：
Deng, HK;Liu, R;Landau, NR
通讯作者：
Landau, NR
HIV-1 感染者外周单核细胞中未整合的环状 HIV-1 DNA：与血浆 HIV-1 RNA 水平高、CD4 计数快速下降以及 AIDS 临床进展相关。
DOI：
10.1097/00042560-199804010-00003
发表时间：
1998
期刊：
Journal of acquired immune deficiency syndromes and human retrovirology : official publication of the International Retrovirology Association
影响因子：
--
作者：
Panther,LA;Coombs,RW;Zeh,JE;Collier,AC;Corey,L
通讯作者：
Corey,L
影响因子：
3.7
作者：
PAUZA, CD;TRIVEDI, P;GRAZIANO, FM
通讯作者：
GRAZIANO, FM
影响因子：
3.7
作者：
Blaak, H;Ran, LJ;Schuitemaker, H
通讯作者：
Schuitemaker, H