The paratransgenic sand fly: a platform for control of Leishmania transmission.
The paratransgenic sand fly: a platform for control of Leishmania transmission.
复制标题
DOI：
10.1186/1756-3305-4-82
复制
发表时间：
2011-05-19
影响因子：
3.2
通讯作者：
Durvasula R
中科院分区：
文献类型：
--
作者：
Hurwitz I;Hillesland H;Fieck A;Das P;Durvasula R
杜氏利什曼原虫是通过白蛉（Phlebotomus argentipes）叮咬传播的。这种寄生虫是内脏利什曼病（VL）的病原体，这是印度比哈尔邦的一种地方病，那里的预防主要依靠喷洒滴滴涕。白蛉种群的农药抗性、环境毒性和有限的资源使这种方法难以实现。一种新的副转基因策略旨在控制L. donovani使用枯草芽孢杆菌（Bacillussubtilis）呈现，枯草芽孢杆菌是从白蛉肠中分离的寄生细菌。在这项工作中，B。将表达绿色荧光蛋白（GFP）的枯草芽孢杆菌添加到灭菌的幼虫食物中。对照盆含有掺有未转化的B的幼虫食物。枯草杆菌或磷酸盐缓冲盐水。将四龄银足拟步行虫幼虫转移到培养基中并使其成熟。测定每个发育阶段的细菌菌落形成单位数、相对丰度和平均微生物负荷。添加B。枯草芽孢杆菌对幼虫食物不影响白蛉羽化率。B。蜡状芽孢杆菌和梭形赖氨酸在每个发育阶段进行鉴定，揭示了内源性微生物的跨静水通道。暴露于外源性B的淋巴细胞。枯草杆菌窝藏显着更大数量的细菌。细菌负荷降低到与来自对照盆的白蛉相当的范围，表明所携带的细菌数量的上限。在含有转化B的幼虫食物中饲养的羽化蝇。枯草杆菌在它们的肠腔中携带大量的这些细菌。在这些副转基因果蝇中检测到强烈的GFP表达，而转化的细菌没有扩散到昆虫的其他隔室。这是第一次证明的副transgenic操纵的P. argentipes。对银足拟步行虫的副转基因操作似乎是可行的。杀利什曼原虫分子通过在银足白蛉繁殖地常见的细菌表达，可以使成年白蛉对利什曼原虫不敏感。Donovani感染
Leishmania donovani is transmitted by the bite of the sand fly, Phlebotomus argentipes. This parasite is the agent of visceral leishmaniasis (VL), an endemic disease in Bihar, India, where prevention has relied mainly on DDT spraying. Pesticide resistance in sand fly populations, environmental toxicity, and limited resources confound this approach. A novel paratransgenic strategy aimed at control of vectorial transmission of L. donovani is presented using Bacillus subtilis, a commensal bacterium isolated from the sand fly gut. In this work, B. subtilis expressing Green Fluorescent Protein (GFP) was added to sterilized larval chow. Control pots contained larval chow spiked either with untransformed B. subtilis or phosphate-buffered saline. Fourth-instar P. argentipes larvae were transferred into the media and allowed to mature. The number of bacterial colony forming units, relative abundance and the mean microbial load were determined per developmental stage. Addition of B. subtilis to larval chow did not affect sand fly emergence rates. B. cereus and Lys fusiformis were identified at each developmental stage, revealing transstadial passage of endogenous microbes. Larvae exposed to an exogenous bolus of B. subtilis harbored significantly larger numbers of bacteria. Bacterial load decreased to a range comparable to sand flies from control pots, suggesting an upper limit to the number of bacteria harbored. Emerging flies reared in larval chow containing transformed B. subtilis carried large numbers of these bacteria in their gut lumens. Strong GFP expression was detected in these paratransgenic flies with no spread of transformed bacteria to other compartments of the insects. This is the first demonstration of paratransgenic manipulation of P. argentipes. Paratransgenic manipulation of P. argentipes appears feasible. Expression of leishmanicidal molecules via commensal bacteria commonly found at breeding sites of P. argentipes could render adult sand flies refractory to L. donovani infection.
登录
查看更多内容
DOI：
10.4269/ajtmh.2008.79.881
发表时间：
2008-12-01
影响因子：
3.3
作者：
Hillesland, Heidi;Read, Amber;Durvasula, Ravi
通讯作者：
Durvasula, Ravi
DOI：
10.1080/00034983.1996.11813102
发表时间：
1996-12-01
影响因子：
--
作者：
Dillon, RJ;ElKordy, E;Lane, RP
通讯作者：
Lane, RP
影响因子：
1.9
作者：
Durvasula, RV;Gumbs, A;Beard, CB
通讯作者：
Beard, CB
中国按蚊群（双翅目：蚊科）：核糖体 DNA 内转录间隔区 2 序列推断的物种区分和系统发育关系
影响因子：
2.1
作者：
Afrane, YA;Lawson, BW;Yan, GY
通讯作者：
Yan, GY
DOI：
10.4269/ajtmh.1959.8.412
发表时间：
1959-01-01
影响因子：
3.3
作者：
GREENBERG, B
通讯作者：
GREENBERG, B