A sex comparison of the anatomy and function of the main olfactory bulb-medial amygdala projection in mice.
A sex comparison of the anatomy and function of the main olfactory bulb-medial amygdala projection in mice.
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DOI：
10.1016/j.neuroscience.2010.11.003
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发表时间：
2011-01-13
期刊：
影响因子：
3.3
通讯作者：
Baum, M. J.
中科院分区：
文献类型：
--
作者：
Kang, N.;McCarthy, E. A.;Cherry, J. A.;Baum, M. J.
我们之前报道过，雌性小鼠的一些主要嗅球（MOB）二尖瓣/簇状（M/T）细胞向“矢状鼻”杏仁核发送直接投射，并选择性地对挥发性雄性小鼠尿液气味做出反应。我们询问在雄性小鼠中是否存在投射到粪鼻扁桃体的MOB M/T细胞，以及这些神经元是否对挥发性雄性尿信息素有两性二态反应。经性腺切除术的雄性和雌性小鼠双侧向内侧杏仁核（Me）注射逆行示踪剂霍乱毒素- b (CTb)，内侧杏仁核是颏鼻扁桃体的一部分。所有受试者在暴露于挥发性男性尿液气味前，均接受苯甲酸雌二醇和黄体酮治疗，90分钟后被处死。对MOB切片进行Fos蛋白和/或CTb免疫染色。与雌性小鼠一样，雄性小鼠也显示出一小部分向母体投射的MOB M/T细胞。虽然这些细胞的一般定位在两性中是相似的，但在被CTb逆行标记的MOB前、后内侧段的MOB M/T细胞的百分比有统计学上的差异。男性尿挥发物刺激了相同的，显著增加Fos表达的MOB M/T神经元在两性中投射到Me。相比之下，在相同的小鼠中，暴露于男性尿液挥发物刺激了仅在女性受试者中副嗅球（AOB）二尖瓣细胞中Fos表达的显著增加。因此，对男性尿液挥发物的任何性别二态行为或神经内分泌反应可能取决于这些气味输入在被主嗅觉系统检测到后，在AOB和/或其他下游前脑结构中的不同处理。
We previously reported that some main olfactory bulb (MOB) mitral/tufted (M/T) cells send a direct projection to the ‘vomeronasal’ amygdala in female mice and selectively respond to volatile male mouse urinary odors. We asked whether MOB M/T cells that project to the vomeronasal amygdala exist in male mice and whether there is a sexually dimorphic response of these neurons to volatile male urinary pheromones. Gonadectomized male and female mice received bilateral injections of the retrograde tracer, Cholera toxin-B (CTb) into the medial amygdala (Me), which is part of the vomeronasal amygdala. All subjects were then treated with estradiol benzoate and progesterone before being exposed to volatile male urinary odors whereupon they were sacrificed 90 min later. Sections of the MOB were immunostained for Fos protein and/or CTb. Male mice, like females, displayed a small population of MOB M/T cells that project to the Me. While the general localization of these cells was similar in the two sexes, there were statistically significant sex differences in the percentage of MOB M/T cells in the anterior and posterior medial segments of the MOB that were retrogradely labeled by CTb. Male urinary volatiles stimulated equivalent, significant increases in Fos expression by MOB M/T neurons projecting to the Me in the two sexes. By contrast, in the same mice exposure to male urinary volatiles stimulated a significant increase in Fos expression by mitral cells in the accessory olfactory bulb (AOB) only in female subjects. Thus any sexually dimorphic behavioral or neuroendocrine responses to male urinary volatiles likely depend on the differential processing of these odor inputs in the AOB and/or other downstream forebrain structures after their detection by the main olfactory system.
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