Intervertebral disc tissue engineering using a novel hyaluronic acid-nanofibrous scaffold (HANFS) amalgam.
Intervertebral disc tissue engineering using a novel hyaluronic acid-nanofibrous scaffold (HANFS) amalgam.
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DOI：
10.1089/ten.tea.2008.0215
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发表时间：
2008-09
影响因子：
--
通讯作者：
Tuan RS
中科院分区：
文献类型：
--
作者：
Nesti LJ;Li WJ;Shanti RM;Jiang YJ;Jackson W;Freedman BA;Kuklo TR;Giuliani JR;Tuan RS
椎间盘退变是一种重大的肌肉骨骼疾病负担。尽管脊柱融合在疼痛管理方面有一定疗效，但脊柱生物力学最终会受到损害。此外，基于硬件的椎间盘置换假体存在固有局限性，这凸显了生物方法修复椎间盘的重要性。在这项研究中，我们将多能的成人骨髓间充质干细胞（MSCs）接种到一种新型生物材料混合物中，以开发一种双相结构，该结构由包裹着透明质酸（HA）水凝胶中心的电纺可生物降解纳米纤维支架（NFS）组成。在转化生长因子 -β存在的情况下，将接种的MSCs在体外诱导进行软骨形成，时间长达28天。软骨性HANFS结构在结构上类似于天然椎间盘，具有外层纤维环（AF）样区域和内层髓核（NP）样区域。组织学和生化分析、免疫组织化学以及基因表达谱分析揭示了接种细胞的软骨细胞表型随时间的发展情况。综上所述，这些发现表明接种MSCs的HANFS结构在退变椎间盘的生物替代组织工程中具有原型潜力。
Degeneration of the intevertebral disc represents a significant musculoskeletal disease burden. Although spinal fusion has some efficacy in pain management, spine biomechanics is ultimately compromised. In addition, there is inherent limitation of hardware-based IVD replacement prostheses, which underscores the importance of biological approaches to disc repair. In this study, we have seeded multipotent, adult human mesenchymal stem cells (MSCs) into a novel biomaterial amalgam to develop a biphasic construct that consisted of electrospun, biodegradable nanofiber scaffold (NFS) enveloping a hyaluronic acid (HA) hydrogel center. The seeded MSCs were induced to undergo chondrogenesis in vitro in the presence of transforming growth factor-β for up to 28 days. The cartilaginous HANFS construct architecturally resembled a native intervertebral disc, with an outer annulus fibrosus (AF)-like region and inner nucleus pulposus (NP)-like region. Histological and biochemical analyses, immunohistochemistry, and gene expression profiling revealed the time-dependent development of chondrocytic phenotype of the seeded cells. Taken together, these findings suggest the prototypic potential of MSC-seeded HANFS constructs for the tissue engineering of biological replacements of degenerated IVD.
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DOI：
10.1016/0304-4165(86)90306-5
发表时间：
1986-09-04
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
FARNDALE, RW;BUTTLE, DJ;BARRETT, AJ
通讯作者：
BARRETT, AJ
影响因子：
14
作者：
Li, WJ;Tuli, R;Tuan, RS
通讯作者：
Tuan, RS
影响因子：
56.9
作者：
LANGER, R;VACANTI, JP
通讯作者：
VACANTI, JP
影响因子：
9.7
作者：
Li, Wan-Ju;Cooper, James A., Jr.;Tuan, Rocky S.
通讯作者：
Tuan, Rocky S.
影响因子：
14
作者：
Li, WJ;Tuli, R;Tuan, RS
通讯作者：
Tuan, RS