Chimeric ferritin nanocages for multiple function loading and multimodal imaging.
Chimeric ferritin nanocages for multiple function loading and multimodal imaging.
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DOI：
10.1021/nl104141g
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发表时间：
2011-02-09
期刊：
影响因子：
10.8
通讯作者：
Chen X
中科院分区：
文献类型：
--
作者：
Lin X;Xie J;Niu G;Zhang F;Gao H;Yang M;Quan Q;Aronova MA;Zhang G;Lee S;Leapman R;Chen X
纳米材料提供了大的表面积，相对于它们的体积，可以在上面加载功能。然而，一个挑战是在加载多个功能时实现精确控制。传统的生物偶联技术随机地以纳米材料的表面官能团为目标，已经发现越来越不足以进行这种控制--这一缺点可能会大大减缓或阻止翻译努力。在目前的研究中，我们评估了铁蛋白纳米笼作为多功能负载的候选纳米平台。铁蛋白纳米笼可以通过基因修饰或化学修饰来赋予其表面功能，而金属阳离子可以通过与金属结合部位的结合而被包裹在其内部。此外，不同类型的铁蛋白纳米笼可以在酸性条件下分解，并在pH为7.4的条件下重新组装，为实现功能杂交提供了一种简便的方法。我们能够利用这些独特性质的组合来生产许多多功能铁蛋白纳米结构，并精确控制它们的组成。然后，我们在体外和体内研究了这些纳米颗粒，以评估它们作为多模式成像探针的潜在适用性。观察到良好的肿瘤靶向性，这归功于增强的渗透性和滞留(EPR)效应和生物载体介导的靶向性。这一点，再加上功能加载技术的通用性，有望使铁蛋白颗粒在纳米医学时代成为一种强大的纳米平台。
Nanomaterials provide large surface areas, relative to their volumes, on which to load functions. One challenge, however, has been to achieve precise control in loading multiple functionalities. Traditional bioconjugation techniques, which randomly target the surface functional groups of nanomaterials, have been found increasingly inadequate for such control--a drawback which may substantially slow down or prohibit the translational efforts. In the current study, we evaluated ferritin nanocages as candidate nanoplatforms for multifunctional loading. Ferritin nanocages can be either genetically or chemically modified to impart functionalities to their surfaces, and metal cations can be encapsulated in their interiors by association with metal binding sites. Moreover, different types of ferritin nanocages can be disassembled under acidic condition and reassembled at pH of 7.4, providing a facile way to achieve function hybridization. We were able to use combinations of these unique properties to produce a number of multifunctional ferritin nanostructures with precise control of their composition. We then studied these nanoparticles, both in vitro and in vivo, to evaluate their potential suitability as multimodality imaging probes. A good tumor targeting profile was observed, which was attributable to both the enhanced permeability and retention (EPR) effect and biovector mediated targeting. This, in combination with the generalizability of the function loading techniques, promises ferritin particles as a powerful nanoplatfom in the era of nanomedicine.
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影响因子：
62.1
作者：
Louie, Angelique Y.
通讯作者：
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DOI：
10.1007/s00259-009-1365-9
发表时间：
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影响因子：
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