Regenerative therapies for equine degenerative joint disease: a preliminary study.
Regenerative therapies for equine degenerative joint disease: a preliminary study.
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DOI：
10.1371/journal.pone.0085917
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Spaas JH
中科院分区：
文献类型：
--
作者：
Broeckx S;Zimmerman M;Crocetti S;Suls M;Mariën T;Ferguson SJ;Chiers K;Duchateau L;Franco-Obregón A;Wuertz K;Spaas JH
退行性关节病（DJD）是马表演者运动功能下降和退休的主要原因。出于这个原因，DJD的再生疗法获得了越来越多的关注。富血小板血浆（PRP）和间充质干细胞（MSC）分离自6岁的供体马。MSC以其天然状态或在软骨形成诱导后使用。在最初的研究中，将20匹在球节关节中具有天然发生的DJD的马分成4组，并注射以下物质：1）PRP; 2）MSC; 3）MSC和PRP;或4）软骨形成诱导的MSC和PRP。然后在注射后6周（T1）、12周（T2）、6个月（T3）和12个月（T4）后通过临床评分系统对马进行评价。在第二项研究中，将30匹具有相同医学背景的马随机分配至两种联合治疗之一，并在T1时进行评价。发现天然MSC的蛋白质表达谱对于主要组织相容性（MHC）II和p63是阴性的，对于Ki 67、胶原II型（Col II）和波形蛋白是低的，对于MHC I是阳性的。软骨形成诱导导致聚集蛋白聚糖、Col II和软骨寡聚基质蛋白（COMP）的mRNA表达增加，以及p63和糖胺聚糖的蛋白表达增加，但Ki 67的蛋白表达减少。与单独PRP治疗相比，PRP和MSC的联合使用显著改善了治疗后6周至12个月受损关节的功能和可持续性。最高的短期临床演变得分获得软骨诱导的MSC和PRP。本研究报告了成功的体外诱导马骨髓间充质干细胞的软骨形成。在患有DJD的马的球节关节中体内应用（诱导的）MSC与PRP一起导致显著的临床改善，直到治疗后12个月。
Degenerative joint disease (DJD) is a major cause of reduced athletic function and retirement in equine performers. For this reason, regenerative therapies for DJD have gained increasing interest. Platelet-rich plasma (PRP) and mesenchymal stem cells (MSCs) were isolated from a 6-year-old donor horse. MSCs were either used in their native state or after chondrogenic induction. In an initial study, 20 horses with naturally occurring DJD in the fetlock joint were divided in 4 groups and injected with the following: 1) PRP; 2) MSCs; 3) MSCs and PRP; or 4) chondrogenic induced MSCs and PRP. The horses were then evaluated by means of a clinical scoring system after 6 weeks (T1), 12 weeks (T2), 6 months (T3) and 12 months (T4) post injection. In a second study, 30 horses with the same medical background were randomly assigned to one of the two combination therapies and evaluated at T1. The protein expression profile of native MSCs was found to be negative for major histocompatibility (MHC) II and p63, low in MHC I and positive for Ki67, collagen type II (Col II) and Vimentin. Chondrogenic induction resulted in increased mRNA expression of aggrecan, Col II and cartilage oligomeric matrix protein (COMP) as well as in increased protein expression of p63 and glycosaminoglycan, but in decreased protein expression of Ki67. The combined use of PRP and MSCs significantly improved the functionality and sustainability of damaged joints from 6 weeks until 12 months after treatment, compared to PRP treatment alone. The highest short-term clinical evolution scores were obtained with chondrogenic induced MSCs and PRP. This study reports successful in vitro chondrogenic induction of equine MSCs. In vivo application of (induced) MSCs together with PRP in horses suffering from DJD in the fetlock joint resulted in a significant clinical improvement until 12 months after treatment.
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影响因子：
2.8
作者：
Frisbie, David D.;Kisiday, John D.;Mcllwraith, C. Wayne
通讯作者：
Mcllwraith, C. Wayne
DOI：
10.1055/s-0038-1632990
发表时间：
2006-01-01
影响因子：
1.3
作者：
Frisbie, D. D.;Cross, M. W.;McIlwraith, C. W.
通讯作者：
McIlwraith, C. W.
DOI：
10.3415/vcot-08-10-0107
发表时间：
2009-01-01
影响因子：
1.3
作者：
Berg, L. C.;Koch, T. G.;Betts, D. H.
通讯作者：
Betts, D. H.
肌腱干细胞和富含血小板的血浆在肌腱愈合中的协同作用
影响因子：
2.8
作者：
Chen, Lei;Dong, Shi-Wu;Xu, Jian-Zhong
通讯作者：
Xu, Jian-Zhong
影响因子：
1.3
作者：
Fang, Baijun;Song, Yongping;Ma, Yuanfang
通讯作者：
Ma, Yuanfang