Isolation and Characterization of a Novel Jumbo Phage from Leaf Litter Compost and Its Suppressive Effect on Rice Seedling Rot Diseases.
Isolation and Characterization of a Novel Jumbo Phage from Leaf Litter Compost and Its Suppressive Effect on Rice Seedling Rot Diseases.
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DOI：
10.3390/v13040591
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发表时间：
2021-03-31
期刊：
影响因子：
--
通讯作者：
Takahashi H
中科院分区：
文献类型：
--
作者：
Sasaki R;Miyashita S;Ando S;Ito K;Fukuhara T;Takahashi H
巨型噬菌体是由二十面体的头部、尾部和其他吸附结构组成的大病毒粒子，其DNA基因组大于200kbp，是自然界中丰富的生物物质。在本研究中，对枯叶堆肥中的噬菌体进行了筛选，研究了它们对由枯草杆菌引起的水稻幼苗腐烂病的抑制作用，并在枯叶堆肥富含滤液的悬浮液中鉴定出一种新的噬菌体。噬菌体颗粒由一个刚性的尾巴二十面体头部组成，包含一个227,105bp的DNA基因组。该噬菌体可裂解5株绿色芽胞杆菌和6株植物伯克霍尔德氏菌。该噬菌体命名为巨型伯克霍尔德氏菌噬菌体FLC6。蛋白质组树分析表明，FLC6噬菌体与两个巨型拉尔斯特菌噬菌体RSF1和RSL2属于清迈病毒属(Myoviridae；Caudovirales)的成员。有趣的是，FLC6还能溶解引起青枯病的两个菌株--拟青枯病病原菌，这表明FLC6具有广泛的寄主范围，这可能使其作为生物防治重要经济作物上的几种细菌病害特别有利。新型巨噬菌体FLC6可使落叶层堆肥抑制多种细菌性病害，其本身也可用于作物栽培中的植物病害防治。
Jumbo phages have DNA genomes larger than 200 kbp in large virions composed of an icosahedral head, tail, and other adsorption structures, and they are known to be abundant biological substances in nature. In this study, phages in leaf litter compost were screened for their potential to suppress rice seedling rot disease caused by the bacterium Burkholderia glumae, and a novel phage was identified in a filtrate-enriched suspension of leaf litter compost. The phage particles consisted of a rigid tailed icosahedral head and contained a DNA genome of 227,105 bp. The phage could lyse five strains of B. glumae and six strains of Burkholderia plantarii. The phage was named jumbo Burkholderia phage FLC6. Proteomic tree analysis revealed that phage FLC6 belongs to the same clade as two jumbo Ralstonia phages, namely RSF1 and RSL2, which are members of the genus Chiangmaivirus (family: Myoviridae; order: Caudovirales). Interestingly, FLC6 could also lyse two strains of Ralstonia pseudosolanacearum, the causal agent of bacterial wilt, suggesting that FLC6 has a broad host range that may make it especially advantageous as a bio-control agent for several bacterial diseases in economically important crops. The novel jumbo phage FLC6 may enable leaf litter compost to suppress several bacterial diseases and may itself be useful for controlling plant diseases in crop cultivation.
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影响因子：
5.6
作者：
Álvarez B;Biosca EG
通讯作者：
Biosca EG
DOI：
10.1099/00207713-37-2-144
发表时间：
1987-04-01
期刊：
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
影响因子：
--
作者：
AZEGAMI, K;NISHIYAMA, K;FUKAZAWA, C
通讯作者：
FUKAZAWA, C
影响因子：
5.2
作者：
Matsui T;Yoshikawa G;Mihara T;Chatchawankanphanich O;Kawasaki T;Nakano M;Fujie M;Ogata H;Yamada T
通讯作者：
Yamada T
DOI：
10.21775/9781910190852.07
发表时间：
2018-01-01
期刊：
VIRUSES OF MICROORGANISMS
影响因子：
--
作者：
Lehman, Susan M.
通讯作者：
Lehman, Susan M.
影响因子：
2.1
作者：
Meczker, Katalin;Doemoetoer, Dora;Kovacs, Tamas
通讯作者：
Kovacs, Tamas