Synthesis and functionalization of monodisperse near-ultraviolet and visible excitable multifunctional Eu(3+), Bi(3+):REVO4 nanophosphors for bioimaging and biosensing applications.
Synthesis and functionalization of monodisperse near-ultraviolet and visible excitable multifunctional Eu(3+), Bi(3+):REVO4 nanophosphors for bioimaging and biosensing applications.
复制标题
DOI：
10.1039/c6nr03369e
复制
发表时间：
2016-06-16
期刊：
影响因子：
6.7
通讯作者：
Parak WJ
中科院分区：
文献类型：
--
作者：
Escudero A;Carrillo-Carrión C;Zyuzin MV;Ashraf S;Hartmann R;Núñez NO;Ocaña M;Parak WJ
基于稀土钒酸盐（REVO4、RE = Y、Gd）的近紫外和可见光可激发 Eu 和 Bi 掺杂纳米粒子可用于生物成像和生物传感应用。基于稀土钒酸盐 (REVO4, RE = Y, Gd) 的近紫外和可见光可激发的 Eu 和 Bi 掺杂纳米粒子已通过简单的路线由适当的 RE 前体、硝酸铕和硝酸铋以及原钒酸钠通过在 120 °C 的乙二醇/水混合物中均匀沉淀来合成。纳米粒子可以通过聚丙烯酸（PAA）的一锅合成或通过聚（烯丙胺盐酸盐）（PAH）和PAA的逐层方法进行功能化。在第一种情况下，也可以通过调节PAA的量来调节粒径。基于Eu-Bi掺杂REVO4的纳米荧光粉表现出典型的Eu(iii)红色发光，它可以通过钒酸根阴离子的能量转移过程被激发，与铕阳离子的直接激发相比，产生更高的发光强度。将 Bi 纳入 REVO4 结构中，使钒酸根阴离子的原始吸收带向更长的波长移动，从而产生在 342 nm 处激发最大值的纳米荧光粉，也可以在可见光范围内激发。此类纳米磷光体在生物成像和生物传感应用中的适用性，以及它们在不同生物缓冲介质中的胶体稳定性、它们的细胞毒性、它们在低pH下的降解性以及它们被HeLa细胞的摄取都得到了评估。还证明了它们对生物成像和生物传感应用的适用性。
Near-ultraviolet and visible excitable Eu- and Bi-doped NPs based on rare earth vanadates (REVO4, RE = Y, Gd) can be used for bioimaging and biosensing applications. Near-ultraviolet and visible excitable Eu- and Bi-doped NPs based on rare earth vanadates (REVO4, RE = Y, Gd) have been synthesized by a facile route from appropriate RE precursors, europium and bismuth nitrate, and sodium orthovanadate, by homogeneous precipitation in an ethylene glycol/water mixture at 120 °C. The NPs can be functionalized either by a one-pot synthesis with polyacrylic acid (PAA) or by a Layer-by-Layer approach with poly(allylamine hydrochloride) (PAH) and PAA. In the first case, the particle size can also be tuned by adjusting the amount of PAA. The Eu- Bi-doped REVO4 based nanophosphors show the typical red luminescence of Eu(iii), which can be excited through an energy transfer process from the vanadate anions, resulting in a much higher luminescence intensity in comparison to the direct excitation of the europium cations. The incorporation of Bi into the REVO4 structure shifts the original absorption band of the vanadate anions towards longer wavelengths, giving rise to nanophosphors with an excitation maximum at 342 nm, which can also be excited in the visible range. The suitability of such nanophosphors for bioimaging and biosensing applications, as well as their colloidal stability in different buffer media of biological interest, their cytotoxicity, their degradability at low pH, and their uptake by HeLa cells have been evaluated. Their suitability for bioimaging and biosensing applications is also demonstrated.
登录
查看更多内容
影响因子：
17.1
作者：
Kastl, Lena;Sasse, Daniel;Rivera Gil, Pilar
通讯作者：
Rivera Gil, Pilar
影响因子：
4.1
作者：
Chen, Yi-Chin;Huang, Sheng-Cih;Chen, Teng-Ming
通讯作者：
Chen, Teng-Ming
影响因子：
17.3
作者：
Gao, XH;Nie, SM
通讯作者：
Nie, SM
DOI：
10.1007/978-94-017-8739-0_5
发表时间：
2014-01-01
期刊：
NANOMATERIAL: IMPACTS ON CELL BIOLOGY AND MEDICINE
影响因子：
--
作者：
Adjei, Isaac M.;Sharma, Blanka;Labhasetwar, Vinod
通讯作者：
Labhasetwar, Vinod
影响因子：
3.9
作者：
Fratila, Raluca M.;Mitchell, Scott G.;de la Fuente, Jesus M.
通讯作者：
de la Fuente, Jesus M.