Effects of lead exposure on blood electrical impedance spectroscopy of mice.
Effects of lead exposure on blood electrical impedance spectroscopy of mice.
复制标题
铅暴露对小鼠血液阻抗谱的影响。
DOI：
10.1186/s12938-021-00933-0
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发表时间：
2021-10-07
影响因子：
3.9
通讯作者：
Ma Q
中科院分区：
文献类型：
--
作者：
Yang B;Xu J;Hu S;You B;Ma Q
铅是一种非必需重金属，可抑制血红素合成，具有显著的细胞毒作用。然而，其对红细胞电学特性的影响尚不清楚。因此，本研究旨在利用电阻抗谱（EIS）在0.01 ~ 100 MHz频率范围内研究铅暴露在小鼠血液中的电学特性和电生理机制。利用阻抗谱数据特征、Bodes图、Nyquist图和Nichols图以及恒相元（Constant Phase Element， CPE）等效电路模型，明确分析血液电阻抗特性的幅频、相频和频率特性的差异。与对照组健康血液相比，铅对血液的影响如下：(1)红细胞压积降低；（ii）电阻抗幅频特性和相频特性降低；（iii）特征频率（f0）显著增加；(4)血浆、红细胞膜、血红蛋白的电阻抗降低，电导率升高。(v)细胞膜伪电容（CPE_Tm）和胞内伪电容（CPE-Ti）降低。因此，EIS可作为监测铅暴露引起的血液和红细胞异常的有效方法。电池的电学特性可作为评价重金属毒性作用的重要观察指标。
Lead is a nonessential heavy metal, which can inhibit heme synthesis and has significant cytotoxic effects. Nevertheless, its effect on the electrical properties of red blood cells (RBCs) remains unclear. Consequently, this study aimed to investigate the electrical properties and the electrophysiological mechanism of lead exposure in mouse blood using Electrical Impedance Spectroscopy (EIS) in 0.01–100 MHz frequency range. Data characteristic of the impedance spectrum, Bodes plot, Nyquist plot and Nichols plot, and Constant Phase Element (CPE) equivalent circuit model were used to explicitly analyze the differences in amplitude–frequency, phase–frequency, and the frequency characteristics of blood in electrical impedance properties. Compared with the healthy blood in control mice, the changes in blood exposed to lead were as follows: (i) the hematocrit decreased; (ii) the amplitude–frequency and phase–frequency characteristics of electrical impedance decreased; (iii) the characteristic frequencies (f0) were significantly increased; (iv) the electrical impedance of plasma, erythrocyte membrane, and hemoglobin decreased, while the conductivity increased. (v) The pseudo-capacitance of cell membrane (CPE_Tm) and the intracellular pseudo-capacitance (CPE-Ti) were decreased. Therefore, EIS can be used as an effective method to monitor blood and RBC abnormalities caused by lead exposure. The electrical properties of the cells can be applied as an important observation in the evaluation of the toxic effects of heavy metals.
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DOI：
10.3390/e22101117
发表时间：
2020-10-03
期刊：
Entropy (Basel, Switzerland)
影响因子：
--
作者：
Herencsar N;Freeborn TJ;Kartci A;Cicekoglu O
通讯作者：
Cicekoglu O
DOI：
10.1016/0302-4598(96)05064-7
发表时间：
1996-08-01
期刊：
BIOELECTROCHEMISTRY AND BIOENERGETICS
影响因子：
--
作者：
Coster, HGL;Chilcott, TC;Coster, ACF
通讯作者：
Coster, ACF
影响因子：
1.2
作者：
Kaneko, Masahiko;Kazatani, Takurou;Shikata, Hisaharu
通讯作者：
Shikata, Hisaharu
影响因子：
11.8
作者：
Kuang, Wenjie;Chen, Zhaofang;Bi, Jun
通讯作者：
Bi, Jun
影响因子：
5.6
作者：
Alves Oliveira AC;Dionizio A;Teixeira FB;Bittencourt LO;Nonato Miranda GH;Oliveira Lopes G;Varela ELP;Nabiça M;Ribera P;Dantas K;Leite A;Buzalaf MAR;Monteiro MC;Maia CSF;Lima RR
通讯作者：
Lima RR