Hippocampal Impairment Triggered by Long-Term Lead Exposure from Adolescence to Adulthood in Rats: Insights from Molecular to Functional Levels.
Hippocampal Impairment Triggered by Long-Term Lead Exposure from Adolescence to Adulthood in Rats: Insights from Molecular to Functional Levels.
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DOI：
10.3390/ijms21186937
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发表时间：
2020-09-21
影响因子：
5.6
通讯作者：
Lima RR
中科院分区：
文献类型：
--
作者：
Alves Oliveira AC;Dionizio A;Teixeira FB;Bittencourt LO;Nonato Miranda GH;Oliveira Lopes G;Varela ELP;Nabiça M;Ribera P;Dantas K;Leite A;Buzalaf MAR;Monteiro MC;Maia CSF;Lima RR
铅（Pb）是长期接触后的一种环境和职业神经毒物。本研究旨在探讨全身铅暴露对大鼠从青春期到成年期的影响，评估海马的分子、形态和功能方面。为此，雄性 Wistar 大鼠通过灌胃暴露于 50 mg/kg 的醋酸铅或蒸馏水 55 天。为了评估短期和长期记忆，进行了物体识别和降压抑制性回避测试。在行为测试结束时，对动物实施安乐死，并对海马进行解剖和处理，以评估： 海马实质中的 Pb 含量水平； Trolox 当量抗氧化能力 (TEAC)、谷胱甘肽 (GSH) 和丙二醛 (MDA) 水平作为氧化应激和抗氧化状态的参数；通过抗 NeuN 免疫组织化学分析进行整体蛋白质组学分析和神经元变性。我们的研究结果表明，成年大鼠从青春期开始接触铅，其海马体内的铅水平增加，丙二醛和谷胱甘肽水平增加，与神经结构和生理相关的蛋白质受到调节，神经元密度降低，因此短期和长期记忆的认知能力较差。那么，在这一时期长期接触铅可能会损害与功能损伤相关的海马结构的几个生物组织水平。
Lead (Pb) is an environmental and occupational neurotoxicant after long-term exposure. This study aimed to investigate the effects of systemic Pb exposure in rats from adolescence to adulthood, evaluating molecular, morphologic and functional aspects of hippocampus. For this, male Wistar rats were exposed to 50 mg/kg of Pb acetate or distilled water for 55 days by intragastric gavage. For the evaluation of short-term and long-term memories, object recognition and step-down inhibitory avoidance tests were performed. At the end of the behavioral tests, the animals were euthanized and the hippocampus dissected and processed to the evaluation of: Pb content levels in hippocampal parenchyma; Trolox equivalent antioxidant capacity (TEAC), glutathione (GSH) and malondialdehyde (MDA) levels as parameters of oxidative stress and antioxidant status; global proteomic profile and neuronal degeneration by anti-NeuN immunohistochemistry analysis. Our results show the increase of Pb levels in the hippocampus of adult rats exposed from adolescence, increased MDA and GSH levels, modulation of proteins related to neural structure and physiology and reduced density of neurons, hence a poor cognitive performance on short and long-term memories. Then, the long-term exposure to Pb in this period of life may impair several biologic organizational levels of the hippocampal structure associated with functional damages.
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影响因子：
4.3
作者：
Chen, HH;Ma, TG;Ho, IK
通讯作者：
Ho, IK
影响因子：
2.7
作者：
ENNACEUR, A;DELACOUR, J
通讯作者：
DELACOUR, J
硫化铅纳米粒子暴露对大鼠海马神经元钙稳态的影响
影响因子：
3.9
作者：
Cao, Yanhua;Liu, Huajie;Cai, Yuan
通讯作者：
Cai, Yuan
影响因子：
4.6
作者：
Dionizio AS;Melo CGS;Sabino-Arias IT;Ventura TMS;Leite AL;Souza SRG;Santos EX;Heubel AD;Souza JG;Perles JVCM;Zanoni JN;Buzalaf MAR
通讯作者：
Buzalaf MAR
DOI：
10.1093/bioinformatics/btp101
发表时间：
2009-04-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Bindea G;Mlecnik B;Hackl H;Charoentong P;Tosolini M;Kirilovsky A;Fridman WH;Pagès F;Trajanoski Z;Galon J
通讯作者：
Galon J