Phylogenetic and Functional Diversity of Saprolegniales and Fungi Isolated from Temperate Lakes in Northeast Germany.
Phylogenetic and Functional Diversity of Saprolegniales and Fungi Isolated from Temperate Lakes in Northeast Germany.
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DOI：
10.3390/jof7110968
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发表时间：
2021-11-13
期刊：
影响因子：
--
通讯作者：
Grossart HP
中科院分区：
文献类型：
--
作者：
Masigol H;Woodhouse JN;van West P;Mostowfizadeh-Ghalamfarsa R;Rojas-Jimenez K;Goldhammer T;Khodaparast SA;Grossart HP
近年来，真菌在淡水生态系统中对植物凋落物降解和溶解性有机物（特别是腐殖物质）转化的作用越来越受到人们的关注。然而，作为最常见的真核生物之一，水霉目的作用很少被研究。在这项研究中，我们从12个德国湖泊中分离出51种真菌菌株和62种水霉目菌株，并对其进行了系统发育分析。我们研究了纤维素，木质素，几丁质分解活性的菌株，使用平板测定。此外，我们使用Biolog FF MicroPlates™测定了10种选定菌株利用95种不同不稳定化合物的能力。最后，三个选定的菌株利用麦芽糖和降解/产生腐殖物质的能力进行了测量。枝孢属和青霉属是其中最普遍的真菌菌株，而水霉属、Achlya和Leptolegnia是最常见的水霉目菌株。虽然被分配到属的分离菌株在遗传学上是相似的，但它们的酶活性和生理特征是相当不同的。我们的研究结果表明，水霉目，与真菌相反，缺乏木质素分解活性，不参与腐殖物质的生产/转化。我们推测，水霉目和真菌可能有互补的作用，在相互作用与溶解的有机质，这对淡水生态系统中的碳循环具有生态意义。
The contribution of fungi to the degradation of plant litter and transformation of dissolved organic matter (humic substances, in particular) in freshwater ecosystems has received increasing attention recently. However, the role of Saprolegniales as one of the most common eukaryotic organisms is rarely studied. In this study, we isolated and phylogenetically placed 51 fungal and 62 Saprolegniales strains from 12 German lakes. We studied the cellulo-, lignino-, and chitinolytic activity of the strains using plate assays. Furthermore, we determined the capacity of 10 selected strains to utilize 95 different labile compounds, using Biolog FF MicroPlates™. Finally, the ability of three selected strains to utilize maltose and degrade/produce humic substances was measured. Cladosporium and Penicillium were amongst the most prevalent fungal strains, while Saprolegnia, Achlya, and Leptolegnia were the most frequent Saprolegniales strains. Although the isolated strains assigned to genera were phylogenetically similar, their enzymatic activity and physiological profiling were quite diverse. Our results indicate that Saprolegniales, in contrast to fungi, lack ligninolytic activity and are not involved in the production/transformation of humic substances. We hypothesize that Saprolegniales and fungi might have complementary roles in interacting with dissolved organic matter, which has ecological implications for carbon cycling in freshwater ecosystems.
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影响因子：
4.5
作者：
Jiang RH;de Bruijn I;Haas BJ;Belmonte R;Löbach L;Christie J;van den Ackerveken G;Bottin A;Bulone V;Díaz-Moreno SM;Dumas B;Fan L;Gaulin E;Govers F;Grenville-Briggs LJ;Horner NR;Levin JZ;Mammella M;Meijer HJ;Morris P;Nusbaum C;Oome S;Phillips AJ;van Rooyen D;Rzeszutek E;Saraiva M;Secombes CJ;Seidl MF;Snel B;Stassen JH;Sykes S;Tripathy S;van den Berg H;Vega-Arreguin JC;Wawra S;Young SK;Zeng Q;Dieguez-Uribeondo J;Russ C;Tyler BM;van West P
通讯作者：
van West P
影响因子：
1.5
作者：
Feng, Baozhen;Li, Peiqian
通讯作者：
Li, Peiqian
影响因子：
4.4
作者：
Borneman, J;Hartin, RJ
通讯作者：
Hartin, RJ
影响因子：
0.4
作者：
Feng, B. Z.;Li, P. Q.;Yu, X. M.
通讯作者：
Yu, X. M.
影响因子：
--
作者：
Agrawal T;Kotasthane AS
通讯作者：
Kotasthane AS