Changes in primary metabolites and volatile organic compounds in cotton seedling leaves exposed to silver ions and silver nanoparticles revealed by metabolomic analysis.
Changes in primary metabolites and volatile organic compounds in cotton seedling leaves exposed to silver ions and silver nanoparticles revealed by metabolomic analysis.
复制标题
作者：
Yang, Yong;Du, PengMeng;Lai, Wenjie;Yin, Liyan;Ding, Yuanhao;Li, Zhonghua;Hu, Haiyan
在气候变化领域，纳米技术为提高作物产量和确保全球农业系统的可持续性提供了便利的工具。银纳米颗粒(AgNPs)具有优异的生理生化特性，在农业上具有潜在的应用前景，已被广泛研究。然而，人们对AgNPs积累对作物生长和发育的毒害效应的机制表示担忧。本研究采用生理分析和代谢组学综合分析相结合的方法，研究了AgNPs对棉花幼苗的影响。盆栽土壤种植的两周大的棉花幼苗被暴露于5 mg/株的AgNP或0.02 mg/株的Ag+(相当于AgNPs释放的游离Ag+)。采用气相色谱-质谱法(GC-MS)和固相微萃取(SPME)-GC-MS法分别鉴定了主要代谢物和挥发性有机化合物(VOCs)。AgNPs对棉花叶片的光合作用有抑制作用。代谢光谱分析鉴定和定量了棉花叶片中的73种主要代谢物和45种挥发性有机化合物。两种处理均显著改变了植物叶片的代谢产物谱。在主要代谢产物中，AgNPs引起氨基酸、糖和糖醇的显著变化。在挥发性有机化合物中，有13种挥发物，主要是醛、烷烃和萜类化合物，只对AgNPs有反应。综上所述，我们的研究表明，AgNPs对初级代谢产物和VOCs的综合影响不仅归因于Ag+的释放，而且是由于AgNP对棉花叶片的专一性作用。这些结果为了解棉花叶片在暴露于AgNPs后的生理和化学变化提供了重要的知识，并为支持AgNPs在农业上的可持续利用提供了新的见解。
In the area of climate change, nanotechnology provides handy tools for improving crop production and assuring sustainability in global agricultural system. Due to excellent physiological and biochemical properties, silver nanoparticles (AgNPs) have been widely studied for potential use in agriculture. However, there are concerns about the mechanism of the toxic effects of the accumulation of AgNPs on crop growth and development. In this study, the impacts of AgNPs on cotton (Gossypium hirsutum) seedlings were evaluated by integrating physiological and comprehensive metabolomic analyses. Potting-soil-grown, two-week-old cotton seedlings were foliar-exposed to 5 mg/plant AgNP or 0.02 mg/plant Ag+ (equivalent to the free Ag+ released from AgNPs). Primary metabolites and volatile organic compounds (VOCs) were identified by gas chromatography–mass spectrometry (GC-MS) and solid-phase microextraction (SPME) GC-MS, respectively. AgNPs inhibited the photosynthetic capacity of the cotton leaves. The metabolic spectrum analysis identified and quantified 73 primary metabolites and 45 VOCs in cotton leaves. Both treatments significantly changed the metabolite profiles of plant leaves. Among the primary metabolites, AgNPs induced marked changes in amino acids, sugars and sugar alcohols. Among the VOCs, 13 volatiles, mainly aldehydes, alkanes and terpenoids, were specifically altered only in response to AgNPs. In summary, our study showed that the comprehensive influence of AgNPs on primary metabolites and VOCs was not merely attributed to the released Ag+ but was caused by AgNP-specific effects on cotton leaves. These results provide important knowledge about the physiological and chemical changes in cotton leaves upon exposure to AgNPs and offer a new insight for supporting the sustainable use of AgNPs in agriculture.
登录
查看更多内容
影响因子：
11.4
作者：
Jimenez, Elena;Lanza, Beatriz;Albaladejo, Jose
通讯作者：
Albaladejo, Jose
DOI：
10.3390/ijerph16132261
发表时间：
2019-07-01
影响因子：
--
作者：
Khan, Imran;Raza, Muhammad Ali;Huang, Linkai
通讯作者：
Huang, Linkai
DOI：
10.3390/nano10091763
发表时间：
2020-09-07
期刊：
Nanomaterials (Basel, Switzerland)
影响因子：
--
作者：
Castillo-Henríquez L;Alfaro-Aguilar K;Ugalde-Álvarez J;Vega-Fernández L;Montes de Oca-Vásquez G;Vega-Baudrit JR
通讯作者：
Vega-Baudrit JR
影响因子：
3.2
作者：
Ali, Mohammad;Kim, Bosung;Ali, Gul Shad
通讯作者：
Ali, Gul Shad
影响因子：
2.7
作者：
El Gamal, Ahmed Y.;Tohamy, Mohamed R.;Farroh, Khaled Y.
通讯作者：
Farroh, Khaled Y.