In vivo detection and quantification of scalar coupled H NMR resonances
In vivo detection and quantification of scalar coupled H NMR resonances
复制标题
标量耦合 1H NMR 共振的体内检测和定量
DOI：
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
D. Rothman
中科院分区：
文献类型：
--
作者：
R. A. Graaf;D. Rothman
在活体核磁共振中，核磁共振波谱是表征脑组织代谢状态的重要工具。个体代谢物绝对浓度的变化，如乳酸和γ-氨基丁酸，是与特定脑部疾病相关的重要标志。在活体1H核磁共振中对代谢物进行绝对定量并不是一项简单的任务，因为大量代谢物在一个很小的光谱区域内共振，导致严重的光谱重叠。此外，在活体1H核磁共振中，由于需要完全的三维空间定位和出色的水抑制，因此变得复杂。标量耦合自旋系统对活体1H核磁共振序列中典型应用的响应可能特别复杂。本文综述了在活体核磁共振中检测和量化标量耦合自旋系统的复杂性。将描述标量耦合的特征和后果。通过频谱编辑，标量耦合可以智能地用于区分标量耦合共振和重叠(非耦合)共振。然而，在许多情况下，标量耦合引入了由空间局部化和水抑制引起的复杂问题。我们将详细分析活体核磁共振中的典型序列对标量耦合共振的影响。最后，将给出GABA、乳酸和葡萄糖的光谱编辑的例子，以描述在vivo1H核磁共振中涉及的一些实际注意事项。
In vivo1H nuclear magnetic resonance (NMR) spectroscopy is an important tool in characterizing the metabolic status of brain tissue. Changes in absolute concentrations of individual metabolites, like lactate and γ-aminobutyric acid (GABA), are important markers associated with specific brain diseases. The absolute quantification of metabolites by in vivo1H NMR is not a straightforward task, because a large number of metabolites are resonating in a small spectral region leading to severe spectral overlap. Furthermore, in vivo1H NMR is complicated by the need for complete three-dimensional spatial localization and excellent water suppression. The response of scalar-coupled spin systems to typically employed in vivo1H NMR sequences can be especially complicated. This article will review the complications of detecting and quantifying scalar-coupled spin-systems by in vivo1H NMR. The characteristics and consequences of scalar coupling will be described. The scalar coupling can be intelligently used to discriminate scalar-coupled resonances from overlapping (uncoupled) resonances by spectral editing. However, in many cases, the scalar coupling introduces complications arising from spatial localization and water suppression. The effect of typical in vivo1H NMR sequences on scalar-coupled resonances will be analyzed in detail. Finally, examples of spectral editing of GABA, lactate, and glucose will be given to describe some of the practical considerations involved with in vivo1H NMR. © 2001 John Wiley & Sons, Inc. Concepts Magn Reson 13: 32–76, 2001
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影响因子：
2.2
作者：
Pfeuffer, J;Tkác, I;Gruetter, R
通讯作者：
Gruetter, R
影响因子：
17.7
作者：
Behar, KL;Rothman, DL;Krystal, JH
通讯作者：
Krystal, JH
DOI：
10.1073/pnas.90.12.5662
发表时间：
1993-06-15
影响因子：
11.1
作者：
ROTHMAN, DL;PETROFF, OAC;MATTSON, RH
通讯作者：
MATTSON, RH
DOI：
10.1073/pnas.81.20.6330
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
ROTHMAN, DL;BEHAR, KL;SHULMAN, RG
通讯作者：
SHULMAN, RG