International network for comparison of HIV neutralization assays: the NeutNet report II.
International network for comparison of HIV neutralization assays: the NeutNet report II.
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DOI：
10.1371/journal.pone.0036438
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Scarlatti G
中科院分区：
文献类型：
--
作者：
Heyndrickx L;Heath A;Sheik-Khalil E;Alcami J;Bongertz V;Jansson M;Malnati M;Montefiori D;Moog C;Morris L;Osmanov S;Polonis V;Ramaswamy M;Sattentau Q;Tolazzi M;Schuitemaker H;Willems B;Wrin T;Fenyö EM;Scarlatti G
中和抗体为许多病毒感染中疫苗诱导的保护性免疫提供了标记。以此类推，免疫诱导的HIV-1中和抗体可以很好地预测疫苗的有效性。因此，中和抗体的评估至关重要，但由于我们不知道哪种检测方法可以提供保护性免疫措施，因此受到阻碍。一项涉及18个不同实验室的国际合作（NeutNet）先前比较了使用单克隆抗体（mAb）和可溶性CD 4的不同检测方法（I期研究）。在本研究（II期）中，13个实验室对多克隆试剂进行了评价。每个实验室针对代表不同遗传亚型和表型的8种病毒组评价了9种血浆。TriMab是三种mAb的混合物，用作阳性对照，允许在总共9种不同的测定中将结果与I期进行比较。该试验使用在外周血单核细胞（PBMC）中产生的未克隆病毒（病毒亲和性试验，VIA）或在HEK 293 T细胞中从分子克隆或从未克隆病毒产生的Env（gp 160）-假型病毒（假病毒，PSV）。靶细胞包括单核细胞和单循环或多循环感染形式的基因工程细胞系。通过使用一系列测定读数定量感染，包括细胞外或细胞内p24抗原检测、荧光素酶、β-半乳糖苷酶或绿色荧光蛋白（GFP）报告基因表达。使用TriMab，I期和II期试验的8种病毒中有6种的结果基本一致，并证实PSV试验比PBMC更灵敏（p = 0.014）。 与多克隆试剂的比较表明，灵敏度取决于病毒和血浆。在这里，我们进一步证明了依赖于中和试剂和病毒的检测灵敏度的明显差异。与I期研究一致，我们建议平行使用PSV和VIA进行疫苗评价。
Neutralizing antibodies provide markers for vaccine-induced protective immunity in many viral infections. By analogy, HIV-1 neutralizing antibodies induced by immunization may well predict vaccine effectiveness. Assessment of neutralizing antibodies is therefore of primary importance, but is hampered by the fact that we do not know which assay(s) can provide measures of protective immunity. An international collaboration (NeutNet) involving 18 different laboratories previously compared different assays using monoclonal antibodies (mAbs) and soluble CD4 (Phase I study). In the present study (Phase II), polyclonal reagents were evaluated by 13 laboratories. Each laboratory evaluated nine plasmas against an 8 virus panel representing different genetic subtypes and phenotypes. TriMab, a mixture of three mAbs, was used as a positive control allowing comparison of the results with Phase I in a total of nine different assays. The assays used either uncloned virus produced in peripheral blood mononuclear cells (PBMCs) (Virus Infectivity Assays, VIA), or Env (gp160)-pseudotyped viruses (pseudoviruses, PSV) produced in HEK293T cells from molecular clones or from uncloned virus. Target cells included PBMC and genetically engineered cell lines in either single- or multiple-cycle infection format. Infection was quantified by using a range of assay read-outs including extra- or intra-cellular p24 antigen detection, luciferase, beta-galactosidase or green fluorescent protein (GFP) reporter gene expression. Using TriMab, results of Phase I and Phase II were generally in agreement for six of the eight viruses tested and confirmed that the PSV assay is more sensitive than PBMC (p = 0.014). Comparisons with the polyclonal reagents showed that sensitivities were dependent on both virus and plasma. Here we further demonstrate clear differences in assay sensitivities that were dependent on both the neutralizing reagent and the virus. Consistent with the Phase I study, we recommend parallel use of PSV and VIA for vaccine evaluation.
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影响因子：
12.3
作者：
Carpenter AE;Jones TR;Lamprecht MR;Clarke C;Kang IH;Friman O;Guertin DA;Chang JH;Lindquist RA;Moffat J;Golland P;Sabatini DM
通讯作者：
Sabatini DM
影响因子：
3.1
作者：
Heyndrickx, Leo;Vermoesen, Tine;Vanham, Guido
通讯作者：
Vanham, Guido
影响因子：
64.5
作者：
MADDON, PJ;DALGLEISH, AG;AXEL, R
通讯作者：
AXEL, R
国际艾滋病毒中和试验比较网络：NeutNet 报告。
影响因子：
3.7
作者：
Fenyo, Eva Maria;Heath, Alan;Dispinseri, Stefania;Holmes, Harvey;Lusso, Paolo;Zolla-Pazner, Susan;Donners, Helen;Heyndrickx, Leo;Alcami, Jose;Bongertz, Vera;Jassoy, Christian;Malnati, Mauro;Montefiori, David;Moog, Christiane;Morris, Lynn;Osmanov, Saladin;Polonis, Victoria;Sattentau, Quentin;Schuitemaker, Hanneke;Sutthent, Ruengpung;Wrin, Terri;Scarlatti, Gabriella
通讯作者：
Scarlatti, Gabriella
影响因子：
120.7
作者：
BELSHE, RB;GRAHAM, BS;FAST, P
通讯作者：
FAST, P