Microbial communities associated with healthy and White syndrome-affected Echinopora lamellosa in aquaria and experimental treatment with the antibiotic ampicillin.
Microbial communities associated with healthy and White syndrome-affected Echinopora lamellosa in aquaria and experimental treatment with the antibiotic ampicillin.
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DOI：
10.1371/journal.pone.0121780
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Sweet M
中科院分区：
文献类型：
--
作者：
Smith D;Leary P;Craggs J;Bythell J;Sweet M
利用变性梯度凝胶电泳（DGGE）对健康和水族馆白色综合征（WS）影响的珊瑚碎片的原生动物和纤毛虫群落进行了筛选。在健康（H）、脱落组织（ST）、WS影响（WSU）和抗生素处理（WST）样品之间发现16 S rRNA原核多样性的显著差异（R = 0.907，p < 0.001）。虽然在WS受影响的样品中发现了3种弧菌，但其中两种在氨苄青霉素治疗后被消除，但病变继续进展，表明它们在发病机制中起次要或次要作用。第三种弧菌在患病样品中的相对丰度略有增加，在非患病样品中丰富。有趣的是，Tenacibaculum sp在健康和WS受影响的样品之间显示出相对丰度的最大增加，在所有WS受影响和处理的样品中表现出一致的高丰度，这表明Tenacibaculum sp可能是这种情况下发病机制的更可能的候选者。与先前的研究相反，细菌丰度在H、ST、WSU或WST之间没有显著变化（ANOVA，F2，6 = 1.000，p = 0.422）。在H和WSU样本中，抗微生物活性（对哈氏弧菌培养物进行评估）均有限（分别为8.1% ±8.2和8.0% ±2.5），且无显著差异（Kruskal-Wallis，χ2（2）= 3.842，p = 0.146）。所有受WS影响的样本中均存在Philaster sp.、Cohnilembus sp.和Pseudokeronopsis sp.，但健康样本中不存在。纤毛虫在WS中的确切作用尚未确定，但有人提出，它们至少负责在疾病中观察到的整洁的病变边界。
Prokaryotic and ciliate communities of healthy and aquarium White Syndrome (WS)-affected coral fragments were screened using denaturing gradient gel electrophoresis (DGGE). A significant difference (R = 0.907, p < 0.001) in 16S rRNA prokaryotic diversity was found between healthy (H), sloughed tissue (ST), WS-affected (WSU) and antibiotic treated (WST) samples. Although 3 Vibrio spp were found in WS-affected samples, two of these species were eliminated following ampicillin treatment, yet lesions continued to advance, suggesting they play a minor or secondary role in the pathogenesis. The third Vibrio sp increased slightly in relative abundance in diseased samples and was abundant in non-diseased samples. Interestingly, a Tenacibaculum sp showed the greatest increase in relative abundance between healthy and WS-affected samples, demonstrating consistently high abundance across all WS-affected and treated samples, suggesting Tenacibaculum sp could be a more likely candidate for pathogenesis in this instance. In contrast to previous studies bacterial abundance did not vary significantly (ANOVA, F2, 6 = 1.000, p = 0.422) between H, ST, WSU or WST. Antimicrobial activity (assessed on Vibrio harveyi cultures) was limited in both H and WSU samples (8.1% ±8.2 and 8.0% ±2.5, respectively) and did not differ significantly (Kruskal-Wallis, χ2 (2) = 3.842, p = 0.146). A Philaster sp, a Cohnilembus sp and a Pseudokeronopsis sp. were present in all WS-affected samples, but not in healthy samples. The exact role of ciliates in WS is yet to be determined, but it is proposed that they are at least responsible for the neat lesion boundary observed in the disease.
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影响因子：
56.9
作者：
Harvell, CD;Kim, K;Vasta, GR
通讯作者：
Vasta, GR
影响因子：
14.9
作者：
Dereeper A;Guignon V;Blanc G;Audic S;Buffet S;Chevenet F;Dufayard JF;Guindon S;Lefort V;Lescot M;Claverie JM;Gascuel O
通讯作者：
Gascuel O
影响因子：
1.8
作者：
Dalton, S. J.;Godwin, S.;Pereg, L.
通讯作者：
Pereg, L.
影响因子：
3.7
作者：
D'Alvise PW;Lillebø S;Prol-Garcia MJ;Wergeland HI;Nielsen KF;Bergh Ø;Gram L
通讯作者：
Gram L
DOI：
10.1007/bf00408082
发表时间：
1972-01-01
期刊：
ARCHIV FUR MIKROBIOLOGIE
影响因子：
--
作者：
BROCK, TD;BROCK, KM;WEISS, RL
通讯作者：
WEISS, RL