A paper electrode integrated lateral flow immunosensor for quantitative analysis of oxidative stress induced DNA damage.
A paper electrode integrated lateral flow immunosensor for quantitative analysis of oxidative stress induced DNA damage.
复制标题
DOI：
10.1039/c4an00313f
复制
发表时间：
2014-06-07
期刊：
影响因子：
--
通讯作者：
Li CZ
中科院分区：
文献类型：
--
作者：
Zhu X;Shah P;Stoff S;Liu H;Li CZ
建立了一种结合电化学和比色检测的新型装置，用于快速测定DNA氧化损伤生物标志物8-羟基-2′-脱氧鸟苷（8-OHdG）。该装置既具有常规试纸试验速度快、成本低的优点，又具有电化学分析的高可靠性和准确性。在横向流动条上进行竞争性免疫反应，以碳纳米管纸为工作电极，通过计时安培法测定控制线上捕获的8-OHdG。同时，用扫描器测量测线的颜色强度，并用ImageJ软件进行分析。该装置能够通过比色法和电化学方法检测PBS中8-OHdG浓度低至2.07 ng mL - 1和3.11 ng mL - 1。此外，该装置成功地用于检测尿液中的8-OHdG，检测限分别为5.76 ng mL - 1（比色法）和8.85 ng mL - 1（电化学法）。综上所述，该双检测仪可为8-OHdG提供一种快速、直观、定量、可行的检测方法。与基于单一方法的测试相比，这两种方法的集成具有两个主要优点。首先，它可以在同一试验中提供双重信任。其次，通过涉及两种原理不同的方法，集成可以潜在地避免来自一种方法的错误结果。此外，这些方法不需要昂贵的设备或训练有素的人员，认为它适合作为一种简单、经济、便携式的现场试剂盒，在各种临床环境中进行8-OHdG的现场监测。
A novel device combining electrochemical and colorimetric detection is developed for the rapid measurement of 8-hydroxy-2′-deoxyguanosine (8-OHdG), a DNA oxidative damage biomarker. The device takes advantage of the speed and low cost of the conventional strip test as well as the high reliability and accuracy of electrochemical assay. Competitive immunoreactions were performed on the lateral flow strip, and the captured 8-OHdG on the control line was determined by chronoamperometric measurement with carbon nanotubes paper as the working electrode. At the same time, the color intensity of the test line was measured by a scanner and analyzed by the ImageJ software. The device was able to detect 8-OHdG concentrations in PBS as low as 2.07 ng mL−1 by the colorimetric method and 3.11 ng mL−1 by the electrochemical method. Furthermore, the device was successfully utilized to detect 8-OHdG in urine with a detection limit of 5.76 ng mL−1 (colorimetric method) and 8.85 ng mL−1 (electrochemical method), respectively. In conclusion, the integrated device with dual detections can provide a rapid, visual, quantitative and feasible detection method for 8-OHdG. The integration of these two methods holds two major advantages over tests based on single method. Firstly, it can provide double confidence on the same assay. Secondly, by involving two methods that differ in principle, the integration could potentially avoid false results coming from one method. In addition, these methods do not require expensive equipment or trained personnel, deeming it suitable for use as a simple, economical, portable field kit for on-site monitoring of 8-OHdG in a variety of clinical settings.
登录
查看更多内容
影响因子：
6.2
作者：
Dungchai, Wijitar;Chailapakul, Orawon;Henry, Charles S.
通讯作者：
Henry, Charles S.
影响因子：
7.4
作者：
GRABAR, KC;FREEMAN, RG;NATAN, MJ
通讯作者：
NATAN, MJ
影响因子：
12.6
作者：
Li, Chen-zhong;Vandenberg, Katherine;Almeide, Eugenio
通讯作者：
Almeide, Eugenio
影响因子：
2
作者：
Hondroulis, Evangelia;Zhang, Zhiqi;Li, Chen-Zhong
通讯作者：
Li, Chen-Zhong
影响因子：
2.8
作者：
Shah, Pratikkumar;Zhu, Xuena;Li, Chen-Zhong
通讯作者：
Li, Chen-Zhong