Genome-wide identification of BBX gene family and their expression patterns under salt stress in soybean.
Genome-wide identification of BBX gene family and their expression patterns under salt stress in soybean.
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DOI：
10.1186/s12864-022-09068-5
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发表时间：
2022-12-12
期刊：
影响因子：
4.4
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中科院分区：
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--
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BBX 基因在调节各种发育过程和应激反应中发挥着关键作用，这些基因已在许多植物物种中得到鉴定和功能表征。然而，我们对大豆中BBX家族的了解非常有限。在这项研究中，在大豆中鉴定并鉴定了 59 个 BBX 基因，这些基因可在系统发育上分为 5 组。 GmBBXs 在各组之间显示出不同的基因结构和基序组成，并且在每组内表现出相似的基因结构和基序组成。值得注意的是，同线性分析表明片段重复有助于 GmBBX 家族的扩展。此外，我们的RNA-Seq数据表明，59个GmBBX在盐胁迫下表现出不同的转录谱，qRT-PCR分析证实了它们的表达模式。其中，22个GmBBX在盐胁迫下发生转录改变，变化超过两倍，支持GmBBX在大豆耐盐胁迫中发挥重要作用。此外，计算分析表明，GmBBXs 可能与 GmGI3、GmTOE1b、GmCOP1、GmCHI 和 GmCRY 相互作用，而八种类型的转录因子显示出与 GmBBX 基因启动子区域结合的潜力。在大豆中鉴定和表征了 59 个 BBX 基因，它们在盐胁迫下的表达模式和计算分析表明了它们在响应盐胁迫中的功能作用。这些发现将有助于未来大豆BBX基因响应盐胁迫的功能和调控机制的研究。在线版本包含可在 10.1186/s12864-022-09068-5 获取的补充材料。
BBX genes are key players in the regulation of various developmental processes and stress responses, which have been identified and functionally characterized in many plant species. However, our understanding of BBX family was greatly limited in soybean. In this study, 59 BBX genes were identified and characterized in soybean, which can be phylogenetically classified into 5 groups. GmBBXs showed diverse gene structures and motif compositions among the groups and similar within each group. Noticeably, synteny analysis suggested that segmental duplication contributed to the expansion of GmBBX family. Moreover, our RNA-Seq data indicated that 59 GmBBXs showed different transcript profiling under salt stress, and qRT-PCR analysis confirmed their expression patterns. Among them, 22 GmBBXs were transcriptionally altered with more than two-fold changes by salt stress, supporting that GmBBXs play important roles in soybean tolerance to salt stress. Additionally, Computational assay suggested that GmBBXs might potentially interact with GmGI3, GmTOE1b, GmCOP1, GmCHI and GmCRY, while eight types of transcription factors showed potentials to bind the promoter regions of GmBBX genes. Fifty-nine BBX genes were identified and characterized in soybean, and their expression patterns under salt stress and computational assays suggested their functional roles in response to salt stress. These findings will contribute to future research in regard to functions and regulatory mechanisms of soybean BBX genes in response to salt stress. The online version contains supplementary material available at 10.1186/s12864-022-09068-5.
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