Use of the Extended Fujita method for representing the molecular weight and molecular weight distributions of native and processed oat beta-glucans.

Use of the Extended Fujita method for representing the molecular weight and molecular weight distributions of native and processed oat beta-glucans.
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DOI:
10.1038/s41598-018-29997-0
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发表时间:
2018-08-07
期刊:
影响因子:
4.6
通讯作者:
Harding SE
Harding SE
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Channell GA;Adams GG;Lu Y;Gillis RB;Dinu V;Grundy MM;Bajka B;Butterworth PJ;Ellis PR;Mackie A;Ballance S;Harding SE

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β 1-3,1-4葡聚糖(“β-葡聚糖”)是谷物细胞壁的关键组分之一,补充了主要结构组分纤维素。β-葡聚糖也是含有燕麦的食物中可溶性纤维的重要来源,并声称具有其他有益的营养特性,如降低人类的血浆胆固醇。β-葡聚糖功能的关键在于其分子量,并且因为其高多分散性-分子量分布。分析超离心法提供了一种无基质的方法(不需要分离柱或介质)来测定聚合物分子量分布。沉降系数分布转换为分子量分布,通过幂律关系,使用一个既定的程序称为扩展藤田方法。首次建立了幂律关系和扩展藤田方法,并将其应用于一系列天然和加工燕麦β-葡聚糖。考虑将这种方法应用于其他来源的β-葡聚糖。
Beta 1–3, 1–4 glucans (“beta-glucans”) are one of the key components of the cell wall of cereals, complementing the main structural component cellulose. Beta-glucans are also an important source of soluble fibre in foods containing oats with claims of other beneficial nutritional properties such as plasma cholesterol lowering in humans. Key to the function of beta-glucans is their molecular weight and because of their high polydispersity - molecular weight distribution. Analytical ultracentrifugation provides a matrix-free approach (not requiring separation columns or media) to polymer molecular weight distribution determination. The sedimentation coefficient distribution is converted to a molecular weight distribution via a power law relation using an established procedure known as the Extended Fujita approach. We establish and apply the power law relation and Extended Fujita method for the first time to a series of native and processed oat beta-glucans. The application of this approach to beta-glucans from other sources is considered.
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