Degradation and detoxification of the triphenylmethane dye malachite green catalyzed by crude manganese peroxidase from Irpex lacteus F17
Degradation and detoxification of the triphenylmethane dye malachite green catalyzed by crude manganese peroxidase from Irpex lacteus F17
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IrpexlacteusF17粗锰过氧化物酶催化三苯甲烷染料孔雀石绿的降解和解毒
DOI：
10.1007/s11356-016-6164-9
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发表时间：
2016-02
期刊：
影响因子：
--
通讯作者：
Rong Jia
中科院分区：
文献类型：
--
作者：
Xueting Yang;Jinzhao Zheng;Yongming Lu;Rong Jia
利用白色腐菌Irpex lacteus F17制备的锰过氧化物酶（MNP）粗品对三苯甲烷染料孔雀石绿色（MG）进行脱色和脱毒。本研究对这些过程中的关键因素（pH、温度、MG、Mn（2+）、H2 O2、MnP）进行了研究。在最佳条件下，投加66.32 U L（-1）的MnP，反应1h，200 mg L（-1）的MG脱色率可达96%。Km、Vmax和kcat值分别为109.9 μmol L（-1）、152.8 μmol L（-1）min（-1）和44.5 s（-1）。MnP对MG的脱色符合一级反应动力学，动力学速率常数为0.0129h（-1）。UV-vis和UPLC分析显示MG降解。此外，通过LC-TOF-MS鉴定了MnP处理0.5 h期间形成的7种不同中间体。这些降解产物通过MG的N-脱甲基化或MG中C-C双键的氧化裂解通过两种不同的途径产生。基于对细菌和藻类进行的生态毒性分析，证实了由MnP催化系统产生的MG代谢物的毒性明显低于母体化合物。这些研究表明，这种酶系统在水生和陆地环境的清理中具有潜在的用途。
Malachite green (MG), a recalcitrant, carcinogenic, and mutagenic triphenylmethane dye, was decolorized and detoxified using crude manganese peroxidase (MnP) prepared from the white rot fungus Irpex lacteus F17. In this study, the key factors (pH, temperature, MG, Mn(2+), H2O2, MnP) in these processes were investigated. Under optimal conditions, 96 % of 200 mg L(-1) of MG was decolorized when 66.32 U L(-1) of MnP was added for 1 h. The K m, V max, and k cat values were 109.9 μmol L(-1), 152.8 μmol L(-1) min(-1), and 44.5 s(-1), respectively. The decolorization of MG by MnP followed first-order reaction kinetics with a kinetic rate constant of 0.0129 h(-1). UV-vis and UPLC analysis revealed degradation of MG. Furthermore, seven different intermediates formed during the MnP treatment of 0.5 h were identified by LC-TOF-MS. These degradation products were generated via two different routes by either N-demethylation of MG or the oxidative cleavage of the C-C double bond in MG. Based on ecotoxicity analyses performed on bacteria and algae, it was confirmed that MG metabolites produced by the MnP-catalyzed system were appreciably less toxic than the parent compound. These studies indicate the potential use of this enzyme system in the clean-up of aquatic and terrestrial environments.
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影响因子：
1.2
作者：
G. Parshetti;S. Kalme;G. Saratale;S. Govindwar
通讯作者：
G. Parshetti;S. Kalme;G. Saratale;S. Govindwar
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作者：
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影响因子：
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作者：
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影响因子：
13.6
作者：
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