Use of the Cell-Dyn 3500 to predict leukemic cell lineage in peripheral blood of dogs and cats.
Use of the Cell-Dyn 3500 to predict leukemic cell lineage in peripheral blood of dogs and cats.
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使用 Cell-Dyn 3500 预测狗和猫外周血中的白血病细胞谱系。
DOI：
10.1111/j.1939-165x.2002.tb00298.x
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发表时间：
2002
影响因子：
1.2
通讯作者：
Pierce,KennethR
中科院分区：
文献类型：
--
作者：
Fernandes,PeterJ;Modiano,JaimeF;Wojcieszyn,John;Thomas,JenniferS;Benson,PatriciaA;Smith3rd,Roger;Avery,AnneC;Burnett,RobertC;Boone,LauraI;Johnson,MarkC;Pierce,KennethR
背景-形态学和细胞化学是犬和猫白血病分类的基础。自动血液学仪器、免疫表型分析、细胞遗传学、和分子生物学显著提高了我们识别和分类自发性骨髓增生性和淋巴增生性疾病的能力。本研究的目的是评估Cell‐Dyn 3500（CD 3500）提供的基于流式细胞术的光散射模式的实用性。用全自动血细胞分析仪对犬和猫外周血中白血病细胞的谱系进行预测。使用基于CD 3500 CBC输出数据的算法对来自15只狗和6只猫的白血病细胞进行临时分类，随后通过流式细胞仪进行表型分析。酶细胞化学、免疫细胞化学、间接流式细胞术和抗原受体基因重排的分析。结果-该算法在19/21只动物中导致关于白血病细胞（红细胞/巨核细胞潜能、髓细胞白血病、单核细胞白血病、慢性粒细胞白血病、淋巴细胞白血病）的个体发生的正确预测。WBC阻抗计数和WBC光学计数的不匹配结合血液中原始细胞的显微镜评估可用于预测具有红系细胞或巨核细胞潜能的骨髓增生性疾病。白细胞光散射模式使得能够区分髓性白血病（以急性髓单核细胞白血病、急性单核细胞白血病、慢性粒细胞白血病为代表）和淋巴细胞白血病（包括急性和慢性淋巴细胞白血病）。1例急性淋巴细胞白血病被误诊为慢性淋巴细胞白血病。结论-免疫分析可应用于由CD 3500产生的数据，以预测犬和猫外周血中白血病细胞的个体发生。这种快速和定量的技术可用于改善诊断决策，扩大治疗选择，并提高预后的准确性。
Background—Morphology and cytochemistry are the foundation for classification of leukemias in dogs and cats. Advances in automated hematology instrumentation, immunophenotyping, cytogenetics, and molecular biology are significantly improving our ability to recognize and classify spontaneous myeloproliferative and lymphoproliferative disorders.Objective—The purpose of this study was to assess the utility of flow cytometry‐based light scatter patterns provided by the Cell‐Dyn 3500 (CD3500) automated hematology analyzer to predict the lineage of leukemic cells in peripheral blood of dogs and cats.Methods—Leukemic cells from 15 dogs and 6 cats were provisionally classified using an algorithm based on the CD3500 CBC output data and were subsequently phenotyped by enzyme cytochemistry, immunocytochemistry, indirect flow cytometry, and analysis of antigen receptor gene rearrangement.Results—The algorithm led to correct predictions regarding the ontogeny of the leukemic cells (erythroid/megakaryocytic potential, myeloid leukemia, monocytic leukemia, chronic granulocytic leukemia, lymphoid leukemia) in 19/21 animals. Mismatches in the WBC impedance count and the WBC optical count in conjunction with microscopic assessment of blasts in the blood were useful for predicting myeloproliferative disorders with erythroid or megakaryocytic potential. The leukocyte light scatter patterns enabled distinction among myeloid leukemias (represented by acute myelomonocytic leukemia, acute monocytic leukemia, chronic granulocytic leukemia) and lymphocytic leukemias (including acute and chronic lymphocytic leukemias). One case of acute lymphocytic leukemia was misidentified as chronic lymphocytic leukemia.Conclusions—Algorithmic analyses can be applied to data generated by the CD3500 to predict the ontogeny of leukemic cells in the peripheral blood of dogs and cats. This rapid and quantitative technique may be used to improve diagnostic decisions, expand therapeutic choices, and increase prognostic accuracy.
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影响因子：
3.4
作者：
M. Vidriales;A. Órfão;M. López;M. González;A. López;M. García;J. Galende;J. S. San Miguel
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J. S. San Miguel
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DOI：
10.1007/bf00638924
发表时间：
1995
期刊：
Comparative Haematology International
影响因子：
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作者：
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E. Hoover
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影响因子：
6.5
作者：
B. Drewinko;P. Bollinger;C. Brailas;S. Moyle;J. Wyatt;E. Simson;D. Johnston;J. Trujillo
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DOI：
10.1111/j.1365-2257.1997.tb00006.x
发表时间：
1997
期刊：
Clinical and laboratory haematology
影响因子：
--
作者：
J. Iles;J. Henniker
通讯作者：
J. Henniker
细胞化学和免疫表型对急性白血病 FAB 分类的可重复性的贡献。
影响因子：
20.3
作者：
G. Browman;P. Neame;P. Soamboonsrup
通讯作者：
P. Soamboonsrup