Strong inflammatory responses and apoptosis in the oviducts of egg-laying hens caused by genotype VIId Newcastle disease virus.
Strong inflammatory responses and apoptosis in the oviducts of egg-laying hens caused by genotype VIId Newcastle disease virus.
复制标题
DOI：
10.1186/s12917-016-0886-2
复制
发表时间：
2016-11-15
影响因子：
2.6
通讯作者：
Wang J
中科院分区：
文献类型：
--
作者：
Li R;Guo K;Liu C;Wang J;Tan D;Han X;Tang C;Zhang Y;Wang J
纽卡斯尔病病毒（NDV）可严重损害蛋鸡的生殖道，导致产蛋量和品质下降。然而，NDV感染后蛋鸡输卵管严重病理损伤的机制尚未完全阐明。本研究通过病理学观察和病毒检测，探讨了强毒VIId基因型NDV感染蛋鸡输卵管原发病变与病毒载量的相关性。通过检测感染输卵管组织细胞凋亡、免疫相关基因表达及淋巴细胞浸润情况，探讨感染输卵管组织病理变化的可能原因。与对照组相比，NDV感染组鸡输卵管中的病毒载量更高，并出现严重的组织病变和凋亡小体。免疫相关基因，包括TLR 3/7/21、MDA 5、IL-2/6/1β、IFN-β、CXCLi 1/2和CCR 5，在枕骨大肌和子宫中显著上调。IL-2在感染后第5天（dpi）在大胃管中呈现最高的mRNA水平变化（137倍）。感染后，CD 3 + CD 4+和CD 3 + CD 8 α+淋巴细胞浸润输卵管枕大孔。较高的病毒载量被发现与NDV感染母鸡的病理变化和促炎细胞因子的表达升高有关。以上结果表明，VIId型NDV引起蛋鸡输卵管严重病变和细胞凋亡与炎症细胞因子、趋化因子的过度释放和淋巴细胞浸润有关，从而导致输卵管功能障碍和产蛋量下降。
Newcastle disease virus (NDV) can cause serious damage to the reproductive tracts of egg-laying hens and leads to egg production and quality reduction. However, the mechanism of severe pathological damage in the oviducts of egg-laying hens after NDV infection has not been fully elucidated. In this study, the correlation between the primary pathological lesions and viral load in the oviducts of egg-laying hens infected with the velogenic genotype VIId NDV strain was evaluated by pathological observation and virus detection. Subsequently, apoptosis, the expression of immune-related genes and lymphocyte infiltration into the infected oviducts were determined to explore the potential causes of the pathological changes. A higher viral load and severe tissue lesions and apoptotic bodies were observed in the oviduct of NDV-infected hens compared with the control. Immune-related genes, including TLR3/7/21, MDA5, IL-2/6/1β, IFN-β, CXCLi1/2, and CCR5, were significantly upregulated in the magnum and uterus. IL-2 presented the highest mRNA level change (137-fold) at 5 days post infection (dpi) in the magnum. Infection led to CD3+CD4+ and CD3+CD8α+ lymphocyte infiltration into the magnum of the oviduct. A higher viral load was found to be associated with pathological changes and the elevated expression of proinflammatory cytokines in the NDV-infected hens. Our results indicate that the severe lesions and apoptosis in the oviducts of egg-laying hens caused by genotype VIId NDV strains are associated with the excessive release of inflammatory cytokines, chemokines and lymphocyte infiltration, which contribute to the dysfunction of the oviducts and the decrease of egg production in hens.
登录
查看更多内容
DOI：
10.1016/j.cimid.2013.10.003
发表时间：
2014-01-01
影响因子：
2
作者：
Rasoli, Mehdi;Yeap, Swee Keong;Omar, Abdul Rahman
通讯作者：
Omar, Abdul Rahman
影响因子：
1.6
作者：
Ebrahimi, Mohammad Majid;Shahsavandi, Shahla;Shamsara, Mahdi
通讯作者：
Shamsara, Mahdi
影响因子：
1.4
作者：
Sung, HW;Reddy, SM;Fadly, AM
通讯作者：
Fadly, AM
影响因子：
2.9
作者：
Kapczynski, Darrell R.;Afonso, Claudio L.;Miller, Patti J.
通讯作者：
Miller, Patti J.
影响因子：
5
作者：
Ravindra, P. V.;Tiwari, Ashok K.;Chauhan, R. S.
通讯作者：
Chauhan, R. S.