Differences in X-chromosome transcriptional activity and cholesterol metabolism between placentae from swine breeds from Asian and Western origins.
Differences in X-chromosome transcriptional activity and cholesterol metabolism between placentae from swine breeds from Asian and Western origins.
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DOI：
10.1371/journal.pone.0055345
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Piedrahita JA
中科院分区：
文献类型：
--
作者：
Bischoff SR;Tsai SQ;Hardison NE;Motsinger-Reif AA;Freking BA;Nonneman DJ;Rohrer GA;Piedrahita JA
为了深入了解两个猪品种之间的胎盘生理差异，注意到他们不同的生殖性能，即中国梅山和白色复合（WC），我们研究了胎盘组织的基因表达谱，收集在25，45，65，85，和105天的妊娠微阵列。使用线性混合模型，使用错误发现率q值≤0.05，在两个猪品种之间鉴定了总共1，595个差异表达基因。在这些基因中，我们确定了XIST的品种特异性亚型，XIST是一种长的非编码RNA，负责女性X染色体剂量补偿。此外，我们通过DIGMAP探索了胎盘基因表达和染色体定位的相互作用，并鉴定了三条野猪X染色体带（Xq13、Xq21、Xp11），这些带代表了梅山和WC在胎盘发育期间不同的转录活性簇。此外，途径分析确定了胎盘胆固醇运输及其合成的基本品种差异。直接测量胆固醇证实，胆固醇含量显着高于梅山与WC胎盘。总之，这项工作确定了关键的代谢途径，不同的胎盘的两个猪品种的生殖多产性的差异。
To gain insight into differences in placental physiology between two swine breeds noted for their dissimilar reproductive performance, that is, the Chinese Meishan and white composite (WC), we examined gene expression profiles of placental tissues collected at 25, 45, 65, 85, and 105 days of gestation by microarrays. Using a linear mixed model, a total of 1,595 differentially expressed genes were identified between the two pig breeds using a false-discovery rate q-value ≤0.05. Among these genes, we identified breed-specific isoforms of XIST, a long non-coding RNA responsible X-chromosome dosage compensation in females. Additionally, we explored the interaction of placental gene expression and chromosomal location by DIGMAP and identified three Sus scrofa X chromosomal bands (Xq13, Xq21, Xp11) that represent transcriptionally active clusters that differ between Meishan and WC during placental development. Also, pathway analysis identified fundamental breed differences in placental cholesterol trafficking and its synthesis. Direct measurement of cholesterol confirmed that the cholesterol content was significantly higher in the Meishan versus WC placentae. Taken together, this work identifies key metabolic pathways that differ in the placentae of two swine breeds noted for differences in reproductive prolificacy.
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