Study of natural diversity in response to a key pathogenicity regulator of Ralstonia solanacearum reveals new susceptibility genes in Arabidopsis thaliana.
Study of natural diversity in response to a key pathogenicity regulator of Ralstonia solanacearum reveals new susceptibility genes in Arabidopsis thaliana.
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DOI：
10.1111/mpp.13135
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发表时间：
2022-03
影响因子：
4.9
通讯作者：
Vailleau F
中科院分区：
文献类型：
--
作者：
Demirjian C;Razavi N;Desaint H;Lonjon F;Genin S;Roux F;Berthomé R;Vailleau F
青枯雷尔氏菌（Ralstonia solanacearum）革兰氏阴性植物病原性细菌通过将III型效应子（T3 Es）直接易位到宿主细胞中的III型分泌系统（T3 SS）发挥其毒力。T3 E的分泌在翻译后水平上受到辅助蛋白、T3 SS控制蛋白和III型分子伴侣的精细控制。HpaP蛋白是III型分泌底物特异性开关（T3 S4）蛋白之一，先前在拟南芥Col-0登录中被强调为毒力因子。在这项研究中，我们建立了一个全基因组关联分析，以探索两个拟南芥作图种群对hpaP突变体的反应的自然多样性：一个全球收集和一个当地种群。定量遗传变异揭示了两个作图群体中不同的遗传结构，与GMI 1000野生型菌株相比，对hpaP突变体的反应总体延迟。我们已经确定了几个数量性状位点（QTL）与hpaP突变体接种。这些QTL所包含的基因参与了不同的生物学过程，其中一些基因对青枯菌的毒力有重要作用。我们的研究集中在四个候选基因，RKL 1，IRE 3，RACK 1B和PEX 3，确定使用全球收集，并验证其中三个作为易感因素。我们的研究结果表明，研究植物对青枯菌突变体毒力关键调节因子的自然多样性反应是识别病原体直接或间接靶向基因的原始而有力的策略。一项全基因组关联研究，探索拟南芥对青枯雷尔氏菌hpaP突变体的自然多样性：一种识别病原体靶向植物基因的原始策略。
Ralstonia solanacearum gram‐negative phytopathogenic bacterium exerts its virulence through a type III secretion system (T3SS) that translocates type III effectors (T3Es) directly into the host cells. T3E secretion is finely controlled at the posttranslational level by helper proteins, T3SS control proteins, and type III chaperones. The HpaP protein, one of the type III secretion substrate specificity switch (T3S4) proteins, was previously highlighted as a virulence factor on Arabidopsis thaliana Col‐0 accession. In this study, we set up a genome‐wide association analysis to explore the natural diversity of response to the hpaP mutant of two A. thaliana mapping populations: a worldwide collection and a local population. Quantitative genetic variation revealed different genetic architectures in both mapping populations, with a global delayed response to the hpaP mutant compared to the GMI1000 wild‐type strain. We have identified several quantitative trait loci (QTLs) associated with the hpaP mutant inoculation. The genes underlying these QTLs are involved in different and specific biological processes, some of which were demonstrated important for R. solanacearum virulence. We focused our study on four candidate genes, RKL1, IRE3, RACK1B, and PEX3, identified using the worldwide collection, and validated three of them as susceptibility factors. Our findings demonstrate that the study of the natural diversity of plant response to a R. solanacearum mutant in a key regulator of virulence is an original and powerful strategy to identify genes directly or indirectly targeted by the pathogen. A genome‐wide association study to explore the natural diversity of Arabidopsis thaliana in response to the Ralstonia solanacearum hpaP mutant: an original strategy to identify plant genes targeted by the pathogen.
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影响因子：
64.8
作者：
Cheng, Zhenyu;Li, Jian-Feng;Niu, Yajie;Zhang, Xue-Cheng;Woody, Owen Z.;Xiong, Yan;Djonovic, Slavica;Millet, Yves;Bush, Jenifer;McConkey, Brendan J.;Sheen, Jen;Ausubel, Frederick M.
通讯作者：
Ausubel, Frederick M.
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6.9
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Chen, Jin-Gui;Ullah, Hemayet;Jones, Alan M.
通讯作者：
Jones, Alan M.
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56.9
作者：
Alonso, JM;Stepanova, AN;Ecker, JR
通讯作者：
Ecker, JR
影响因子：
4.5
作者：
Brachi B;Faure N;Horton M;Flahauw E;Vazquez A;Nordborg M;Bergelson J;Cuguen J;Roux F
通讯作者：
Roux F
影响因子：
4.9
作者：
Aoun N;Desaint H;Boyrie L;Bonhomme M;Deslandes L;Berthomé R;Roux F
通讯作者：
Roux F