3D imaging of sex-sorted bovine spermatozoon locomotion, head spin and flagellum beating.
3D imaging of sex-sorted bovine spermatozoon locomotion, head spin and flagellum beating.
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DOI：
10.1038/s41598-018-34040-3
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发表时间：
2018-10-23
影响因子：
4.6
通讯作者：
Ozcan A
中科院分区：
文献类型：
--
作者：
Daloglu MU;Lin F;Chong B;Chien D;Veli M;Luo W;Ozcan A
随着精子性别分选方法和计算机辅助精子分析平台的出现，比较二维运动性研究表明，X 分选和 Y 分选精子细胞的游动速度没有显着差异，澄清了早期的误解。然而，由于深度分辨率差以及视场和空间分辨率之间的权衡，传统光学显微镜在揭示自由游动精子细胞的完整 3D 运动方面受到限制，因此它们游泳动力学的其他差异可能无法检测到。使用双视图片上全息显微镜，我们获得了 235 个 X 分类和 289 个 Y 分类牛精子的完整 3D 运动，精确地揭示了它们的 3D 平移头部运动和头部旋转的角速度以及 3D 鞭毛运动。我们的结果证实，X 和 Y 分选的精子群之间的各种运动参数仍然相似。然而，我们发现，Y 分选的牛精子对螺旋形 3D 游泳轨迹的偏好存在统计学上的显着差异，与 X 分选的精子相比，也表现出更高的线性。进一步的研究，例如 X 分类和 Y 分类精子细胞对周围化学物质和离子的运动学反应的差异，可能会更多地阐明这些结果的起源。
With the advent of sperm sex sorting methods and computer-aided sperm analysis platforms, comparative 2D motility studies showed that there is no significant difference in the swimming speeds of X-sorted and Y-sorted sperm cells, clarifying earlier misconceptions. However, other differences in their swimming dynamics might have been undetectable as conventional optical microscopes are limited in revealing the complete 3D motion of free-swimming sperm cells, due to poor depth resolution and the trade-off between field-of-view and spatial resolution. Using a dual-view on-chip holographic microscope, we acquired the full 3D locomotion of 235X-sorted and 289 Y-sorted bovine sperms, precisely revealing their 3D translational head motion and the angular velocity of their head spin as well as the 3D flagellar motion. Our results confirmed that various motility parameters remain similar between X- and Y-sorted sperm populations; however, we found out that there is a statistically significant difference in Y-sorted bovine sperms’ preference for helix-shaped 3D swimming trajectories, also exhibiting an increased linearity compared to X-sorted sperms. Further research on e.g., the differences in the kinematic response of X-sorted and Y-sorted sperm cells to the surrounding chemicals and ions might shed more light on the origins of these results.
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影响因子：
64.8
作者：
ERICSSON, RJ;LANGEVIN, CN;NISHINO, M
通讯作者：
NISHINO, M
影响因子：
2.8
作者：
de Graaf, S. P.;Beilby, K. H.;Maxwell, W. M. C.
通讯作者：
Maxwell, W. M. C.
影响因子：
3.4
作者：
Di Caprio, Giuseppe;El Mallahi, Ahmed;Dubois, Frank
通讯作者：
Dubois, Frank
影响因子：
2.8
作者：
de Graaf, S. P.;Evans, G.;O'Brien, J. K.
通讯作者：
O'Brien, J. K.
DOI：
10.3390/bios5020141
发表时间：
2015-04-01
期刊：
Biosensors
影响因子：
--
作者：
Ferrara MA;Di Caprio G;Managò S;De Angelis A;Sirleto L;Coppola G;De Luca AC
通讯作者：
De Luca AC