Global gene expression shift during the transition from early neural development to late neuronal differentiation in Drosophila melanogaster.
Global gene expression shift during the transition from early neural development to late neuronal differentiation in Drosophila melanogaster.
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DOI：
10.1371/journal.pone.0097703
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Barrio R
中科院分区：
文献类型：
--
作者：
Cantera R;Ferreiro MJ;Aransay AM;Barrio R
转录调控是参与动物发育的机制之一，指导模式化和细胞命运特化的变化。大的时间数据系列，在果蝇的整个生命周期的微阵列的基础上，揭示了存在的基因组的表达增加或减少的时间相关的生命周期。这些基因组富含不同的生物学功能。在这里，而不是使用整个基因组表达数据搜索基因表达的时间重合，我们只在预定义的功能相关基因的目录内搜索基因表达的时间重合，并研究目录的表达谱是否可以用于生成更大的目录，富集相同功能所需的基因。我们分析了已经与早期神经发育和晚期神经分化相关的基因的表达谱，在果蝇生命周期的胚胎阶段16和17。我们假设，在此期间，我们会发现全球下调的基因，早期神经元发育的重要性，以及全球上调的基因所必需的神经元的最终分化。我们的研究结果与这一假设是一致的。然后，我们研究了代表神经发育特定过程的基因目录的表达谱是否与这些过程发生的时间顺序沿着匹配。参与模式化、神经发生、轴突发生或突触传递的基因的谱与预测相匹配，在胚胎中发生相应的生物过程时具有最大的转录值。此外，我们通过在全基因组范围内系统搜索在相应的胚胎间隔具有最高表达水平的基因，获得了富含时间匹配功能的基因的目录。这些发现意味着使用基因表达数据与已知的生物信息相结合，以预测参与特定的生物事件的功能不明的基因。
Regulation of transcription is one of the mechanisms involved in animal development, directing changes in patterning and cell fate specification. Large temporal data series, based on microarrays across the life cycle of the fly Drosophila melanogaster, revealed the existence of groups of genes which expression increases or decreases temporally correlated during the life cycle. These groups of genes are enriched in different biological functions. Here, instead of searching for temporal coincidence in gene expression using the entire genome expression data, we searched for temporal coincidence in gene expression only within predefined catalogues of functionally related genes and investigated whether a catalogue's expression profile can be used to generate larger catalogues, enriched in genes necessary for the same function. We analyzed the expression profiles from genes already associated with early neurodevelopment and late neurodifferentiation, at embryonic stages 16 and 17 of Drosophila life cycle. We hypothesized that during this interval we would find global downregulation of genes important for early neuronal development together with global upregulation of genes necessary for the final differentiation of neurons. Our results were consistent with this hypothesis. We then investigated if the expression profile of gene catalogues representing particular processes of neural development matched the temporal sequence along which these processes occur. The profiles of genes involved in patterning, neurogenesis, axogenesis or synaptic transmission matched the prediction, with largest transcript values at the time when the corresponding biological process takes place in the embryo. Furthermore, we obtained catalogues enriched in genes involved in temporally matching functions by performing a genome-wide systematic search for genes with their highest expression levels at the corresponding embryonic intervals. These findings imply the use of gene expression data in combination with known biological information to predict the involvement of functionally uncharacterized genes in particular biological events.
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影响因子：
4.4
作者：
Ferreiro MJ;Rodríguez-Ezpeleta N;Pérez C;Hackenberg M;Aransay AM;Barrio R;Cantera R
通讯作者：
Cantera R
DOI：
10.1007/bf00848159
发表时间：
1984-01-01
期刊：
WILHELM ROUXS ARCHIVES OF DEVELOPMENTAL BIOLOGY
影响因子：
--
作者：
HARTENSTEIN, V;CAMPOSORTEGA, JA
通讯作者：
CAMPOSORTEGA, JA
影响因子：
64.8
作者：
Graveley BR;Brooks AN;Carlson JW;Duff MO;Landolin JM;Yang L;Artieri CG;van Baren MJ;Boley N;Booth BW;Brown JB;Cherbas L;Davis CA;Dobin A;Li R;Lin W;Malone JH;Mattiuzzo NR;Miller D;Sturgill D;Tuch BB;Zaleski C;Zhang D;Blanchette M;Dudoit S;Eads B;Green RE;Hammonds A;Jiang L;Kapranov P;Langton L;Perrimon N;Sandler JE;Wan KH;Willingham A;Zhang Y;Zou Y;Andrews J;Bickel PJ;Brenner SE;Brent MR;Cherbas P;Gingeras TR;Hoskins RA;Kaufman TC;Oliver B;Celniker SE
通讯作者：
Celniker SE
通过神经胶质调节突触连通性。
影响因子：
64.8
作者：
Eroglu, Cagla;Barres, Ben A.
通讯作者：
Barres, Ben A.
DOI：
10.1016/s1937-6448(10)83003-5
发表时间：
2010
影响因子：
--
作者：
Blauth, Kevin;Banerjee, Swati;Bhat, Manzoor A.
通讯作者：
Bhat, Manzoor A.