Hypolobocera guayaquilensis (Decapoda: Pseudothelphusidae): A New Crab Intermediate Host of Paragonimus mexicanus in Manabí Province, Ecuador.
Hypolobocera guayaquilensis (Decapoda: Pseudothelphusidae): A New Crab Intermediate Host of Paragonimus mexicanus in Manabí Province, Ecuador.
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DOI：
10.3347/kjp.2018.56.2.189
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发表时间：
2018-04
期刊：
影响因子：
--
通讯作者：
Sugiyama H
中科院分区：
文献类型：
--
作者：
Calvopina M;Romero-Alvarez D;Rendon M;Takagi H;Sugiyama H
为了确定并殖吸虫属在厄瓜多尔太平洋地区的热带地区（该地区最近记录了人类肺并殖吸虫病例）的活跃传播，从厄瓜多尔马纳比省足目地区的2条不同溪流中收集了共计75只淡水蟹。根据雄性生殖足的形态特征，所有采集的蟹被鉴定为Hypolobocera guayaquilensis。通过将每只蟹的肝胰腺压在2个玻璃板之间，然后在立体显微镜下观察，检查肝胰腺。在39只（52.0%）蟹体内检出并殖吸虫囊蚴，其密度为1 ~ 32只/只。蟹体大小与囊蚴密度呈正相关。本研究获得的并殖吸虫囊蚴核糖体RNA基因第二内转录间隔区序列与DDBJ/EMBL/GenBank数据库中的墨西哥并殖吸虫核糖体RNA基因第二内转录间隔区序列完全一致。因此，本研究首次证实了中华绒螯蟹H. guayaquilensis是厄瓜多尔马纳比省墨西哥黑腹蛛的第二中间宿主。由于这种螃蟹可能是该地区人类感染的可能来源，居民应注意与一种被忽视的寄生虫病有关的不正确的食蟹习惯，即，并殖吸虫
To determine that Paragonimus sp. is actively transmitted in a tropical area of the Pacific region of Ecuador where human cases of pulmonary paragonimiasis have recently been documented, a total of 75 freshwater crabs were collected from 2 different streams in the Pedernales area of Manabí Province, Ecuador. All collected crabs were identified as Hypolobocera guayaquilensis based on morphological characteristics of the male gonopods. The hepatopancreas of each crab was examined by compressing it between 2 glass plates followed by observation under a stereomicroscope. Excysted Paragonimus metacercariae were detected in 39 (52.0%) crabs and their densities varied from 1 to 32 per infected crab. There was a positive relationship between crab size and metacercarial density. Sequences of the second internal transcribed spacer region of the ribosomal RNA gene of the Paragonimus metacercariae obtained in this study were identical to those of Paragonimus mexicanus deposited in the DDBJ/EMBL/GenBank database. Thus, the present study is the first to confirm that the crab species H. guayaquilensis is the second intermediate host of P. mexicanus in Manabí Province, Ecuador. Because this crab might be the possible source of human infections in this area, residents should pay attention to improper crab-eating habits related with a neglected parasitic disease, i.e., paragonimiasis.
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影响因子：
7.7
作者：
Lopez-Caballero, J.;Oceguera-Figueroa, A.;Leon-Regagnon, V.
通讯作者：
Leon-Regagnon, V.
影响因子：
1.6
作者：
Iwagami, M;Monroy, C;Agatsuma, T
通讯作者：
Agatsuma, T
影响因子：
1.9
作者：
Hernandez-Chea, Roderico;Eugenia Jimenez-Rocha, Ana;Dolz, Gaby
通讯作者：
Dolz, Gaby
影响因子：
2.8
作者：
Calvopiña M;Romero D;Castañeda B;Hashiguchi Y;Sugiyama H
通讯作者：
Sugiyama H
影响因子：
2.4
作者：
Blair, D;Agatsuma, T;Ito, A
通讯作者：
Ito, A