Proteobacteria and Firmicutes Secreted Factors Exert Distinct Effects on Pseudomonas aeruginosa Infection under Normoxia or Mild Hypoxia.
Proteobacteria and Firmicutes Secreted Factors Exert Distinct Effects on Pseudomonas aeruginosa Infection under Normoxia or Mild Hypoxia.
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DOI：
10.3390/metabo12050449
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发表时间：
2022-05-17
期刊：
影响因子：
4.1
通讯作者：
Apidianakis Y
中科院分区：
文献类型：
--
作者：
Charalambous A;Grivogiannis E;Dieronitou I;Michael C;Rahme L;Apidianakis Y
微生物群可能会改变病原体在多种微生物感染部位的毒力潜力。在这里，我们开发了一种多模式果蝇测定，适合在常氧或轻度缺氧下使用果蝇存活或中肠再生作为读数来评估人类细菌相互作用。通过喂养部署由 12 x 33 一对一果蝇共同感染组成的矩阵，我们根据果蝇的存活情况将细菌相互作用分为中性、协同或拮抗。其中 26% 的相互作用是拮抗的，主要发生在变形菌之间。具体而言，铜绿假单胞菌感染可被多种克雷伯氏菌菌株、鲍曼不动杆菌和大肠杆菌拮抗。我们在第二次筛选中验证了这些相互作用，其中基于中肠再生的评估，对 7 x 34 一对一的果蝇共感染进行了评估，并在细菌共培养试管测定中验证了这些相互作用，其中拮抗相互作用取决于高糖可用性时产生的分泌因子。此外，肠球菌在果蝇和试管中与铜绿假单胞菌相互作用，增强铜绿假单胞菌的毒力和绿脓菌素的产生。然而，乳酸菌及其严重缺氧的培养物上清液在苍蝇或小鼠感染铜绿假单胞菌时均未分别提供生存益处。我们认为，在常氧或轻度缺氧的部位，厚壁菌门可能会加剧，而变形杆菌分泌的因子可能会改善铜绿假单胞菌感染。
Microbiota may alter a pathogen’s virulence potential at polymicrobial infection sites. Here, we developed a multi-modal Drosophila assay, amenable to the assessment of human bacterial interactions using fly survival or midgut regeneration as a readout, under normoxia or mild hypoxia. Deploying a matrix of 12 by 33 one-to-one Drosophila co-infections via feeding, we classified bacterial interactions as neutral, synergistic, or antagonistic, based on fly survival. Twenty six percent of these interactions were antagonistic, mainly occurring between Proteobacteria. Specifically, Pseudomonas aeruginosa infection was antagonized by various Klebsiella strains, Acinetobacter baumannii, and Escherichia coli. We validated these interactions in a second screen of 7 by 34 one-to-one Drosophila co-infections based on assessments of midgut regeneration, and in bacterial co-culture test tube assays, where antagonistic interactions depended on secreted factors produced upon high sugar availability. Moreover, Enterococci interacted synergistically with P. aeruginosa in flies and in test tubes, enhancing the virulence and pyocyanin production by P. aeruginosa. However, neither lactic acid bacteria nor their severely hypoxic culture supernatants provided a survival benefit upon P. aeruginosa infection of flies or mice, respectively. We propose that at normoxic or mildly hypoxic sites, Firmicutes may exacerbate, whereas Proteobacteria secreted factors may ameliorate, P. aeruginosa infections.
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DOI：
10.1038/nrmicro3552
发表时间：
2016-01
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
Donaldson GP;Lee SM;Mazmanian SK
通讯作者：
Mazmanian SK
影响因子：
3.7
作者：
Dalton T;Dowd SE;Wolcott RD;Sun Y;Watters C;Griswold JA;Rumbaugh KP
通讯作者：
Rumbaugh KP
影响因子：
2.4
作者：
Galenza A;Hutchinson J;Campbell SD;Hazes B;Foley E
通讯作者：
Foley E
影响因子：
168.9
作者：
Allegranzi, Benedetta;Nejad, Sepideh Bagheri;Pittet, Didier
通讯作者：
Pittet, Didier
影响因子：
4.9
作者：
Chen, Yi-sheng;Wu, Hui-chung;Yanagida, Fujitoshi
通讯作者：
Yanagida, Fujitoshi