Heterodera avenae GLAND5 Effector Interacts With Pyruvate Dehydrogenase Subunit of Plant to Promote Nematode Parasitism

Heterodera avenae GLAND5 Effector Interacts With Pyruvate Dehydrogenase Subunit of Plant to Promote Nematode Parasitism
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燕麦囊肿GLAND5效应子与植物丙酮酸脱氢酶亚基相互作用促进线虫寄生

DOI:
10.3389/fmicb.2019.01241
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发表时间:
2019-06
影响因子:
5.2
通讯作者:
Jian Heng
Jian Heng
中科院分区:
生物学2区
文献类型:
--
作者:
Yang Shanshan;Pan Lingling;Chen Yongpan;Yang Dan;Liu Qian;Jian Heng

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燕麦胞囊线虫主要侵染禾谷类作物,造成严重的经济损失。许多研究表明,寄生线虫可以分泌效应蛋白来抑制植物的免疫反应,进而促进寄生。在这项研究中,我们发现HaGland5,一个新的H。avenae基因在线虫背食道腺细胞中特异表达,在线虫寄生初期表达上调。表达HaGland5的转基因拟南芥株系对H. schachtii比野生型对照植物。相反,通过大麦条纹花叶病毒介导的宿主诱导基因沉默技术沉默HaGland5,显著降低了H.小麦中的燕麦。此外,HaGland5还能抑制植物的防御反应,包括抑制植物防御相关基因的表达、减少细胞壁胼胝质的沉积和活性氧的释放。质谱、免疫共沉淀和萤火虫荧光素酶互补成像分析证实HaGland5与拟南芥丙酮酸脱氢酶亚基(AtEMB3003)特异性相互作用。
Heterodera avenae mainly infects cereal crops and causes severe economic losses. Many studies have shown that parasitic nematodes can secrete effector proteins to suppress plant immune responses and then promote parasitism. In this study, we showed that HaGland5, a novel effector of H. avenae, was exclusively expressed in dorsal esophageal gland cell of nematode, and up-regulated in the early parasitic stage. Transgenic Arabidopsis thaliana lines expressing HaGland5 were significantly more susceptible to H. schachtii than wild-type control plants. Conversely, silencing of HaGland5 through barley stripe mosaic virus-medicated host-induced gene silencing technique substantially reduced the infection of H. avenae in wheat. Moreover, HaGland5 could suppress the plant defense responses, including the repression of plant defense-related genes, reducing deposition of cell wall callose and the burst of reactive oxygen species. Mass spectrometry, co-immunoprecipitation, and firefly luciferase complementation imaging assays confirmed that HaGland5 interacted specifically with Arabidopsis pyruvate dehydrogenase subunit (AtEMB3003).
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发表时间: 2016-06-10
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影响因子: 12.3
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影响因子: --
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发表时间: 2016-02
期刊: The New phytologist
影响因子: --
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DOI: 10.1007/978-94-007-0434-3_8
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