Five mouse tubulin isotypes and their regulated expression during development.
Five mouse tubulin isotypes and their regulated expression during development.
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DOI：
10.1083/jcb.101.3.852
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发表时间：
1985-09
期刊：
影响因子：
--
通讯作者：
Cowan NJ
中科院分区：
文献类型：
--
作者：
Lewis SA;Lee MG;Cowan NJ
我们描述了五个小鼠微管蛋白克隆cDNA，两个（M α 1和M α 2）编码α-微管蛋白，三个（M β 2、M β 4和M β 5）编码β-微管蛋白。这些克隆的序列显示，每个克隆代表不同的基因产物。在两个α-微管蛋白cDNA的共同序列内，编码的氨基酸是相同的，尽管3'非编码区完全不同。相比之下，三种β-微管蛋白cDNA显示出相当大的羧基末端异质性。由克隆序列定义的两种β-微管蛋白同种型在小鼠和人之间是绝对保守的，并且所有三种β-微管蛋白同种型在小鼠和大鼠之间是保守的。这一结果意味着存在的选择性限制，保持物种分化后的序列同一性。第三种小鼠β-微管蛋白同种型和鸡β-微管蛋白同种型之间的近似同一性加强了这一结论。微管蛋白同种型的种间保护的意义进行了讨论微管功能的关系。我们用5个克隆的小鼠微管蛋白cDNA的非交叉杂交3'非编码区探针研究了每种同种型在不同小鼠组织中的发育表达。M α 1和M β 2以近似协调的方式表达，并且它们的转录物在脑和肺中最丰富。M α 2和M β 5在各组织中普遍表达且表达程度相似，在脾脏、胸腺和未成熟脑中丰度最高。相比之下，M β 4仅在大脑中表达。而后者的同种型的表达显着增加在出生后的发展，转录从所有其他四个微管蛋白基因下降，从出生前或出生时的最高水平。微管蛋白同种型特异性mRNA丰度的组织特异性发育变化与小鼠器官发生的关系进行了讨论。
We describe five mouse tubulin cloned cDNAs, two (M alpha 1 and M alpha 2) that encode alpha-tubulin and three (M beta 2, M beta 4, and M beta 5) that encode beta-tubulin. The sequence of these clones reveals that each represents a distinct gene product. Within the sequence common to the two alpha-tubulin cDNAs, the encoded amino acids are identical, though the 3' noncoding regions are wholly dissimilar. In contrast, the three beta-tubulin cDNAs show considerable carboxy-terminal heterogeneity. Two of the beta-tubulin isotypes defined by the cloned sequences are absolutely conserved between mouse and human, and all three beta-tubulin isotypes are conserved between mouse and rat. This result implies the existence of selective constraints that have maintained sequence identity after species divergence. This conclusion is reinforced by the near identity between a third mouse beta-tubulin isotype and a chicken beta-tubulin isotype. The significance of the interspecies conservation of tubulin isotypes is discussed in relationship to microtubule function. We have used non-cross- hybridizing 3' noncoding region probes from the five cloned mouse tubulin cDNAs to study the developmental expression of each isotype in various mouse tissues. M alpha 1 and M beta 2 are expressed in an approximately coordinate fashion, and their transcripts are most abundant in brain and lung. M alpha 2 and M beta 5 are ubiquitously expressed and to a similar extent in each tissue, with the greatest abundance in spleen, thymus, and immature brain. In contrast, M beta 4 is expressed exclusively in brain. Whereas the expression of the latter isotype increases dramatically during postnatal development, transcripts from all four other tubulin genes decline from maximum levels at or before birth. Tissue-specific development changes in the abundance of tubulin isotype-specific mRNAs are discussed in relationship to organogenesis in the mouse.
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影响因子：
14.9
作者：
GINZBURG, I;BEHAR, L;LITTAUER, UZ
通讯作者：
LITTAUER, UZ
DOI：
10.1073/pnas.80.1.31
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
HELFMAN, DM;FERAMISCO, JR;HUGHES, SH
通讯作者：
HUGHES, SH
影响因子：
64.5
作者：
NEFF, NF;THOMAS, JH;BOTSTEIN, D
通讯作者：
BOTSTEIN, D
影响因子：
64.5
作者：
KEMPHUES, KJ;RAFF, EC;KAUFMAN, TC
通讯作者：
KAUFMAN, TC
影响因子：
5.3
作者：
HALL, JL;DUDLEY, L;COWAN, NJ
通讯作者：
COWAN, NJ