Leisingera sp. JC1, a Bacterial Isolate from Hawaiian Bobtail Squid Eggs, Produces Indigoidine and Differentially Inhibits Vibrios.
Leisingera sp. JC1, a Bacterial Isolate from Hawaiian Bobtail Squid Eggs, Produces Indigoidine and Differentially Inhibits Vibrios.
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DOI：
10.3389/fmicb.2016.01342
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发表时间：
2016
影响因子：
5.2
通讯作者：
Balunas MJ
中科院分区：
文献类型：
--
作者：
Gromek SM;Suria AM;Fullmer MS;Garcia JL;Gogarten JP;Nyholm SV;Balunas MJ
许多头足类物种的雌性成员在生殖系统的附属腺(ANG)中有一个细菌联合体。这些细菌被沉积到卵中，然后在环境中产卵，在那里它们必须不受捕食、病原体和污垢的保护。在本研究中，我们对Leisingera sp.的基因组和次生代谢物的产生进行了研究。Jc1，从夏威夷短尾乌贼(Euprymna Scolope)的卵的凝胶层中分离出来的甲型变态杆菌的玫瑰杆菌分支(红杆菌科)的成员。全基因组测序和MLSA分析表明，Leisingera sp.Jc1属于与鱿鱼血管生成素相关的一组玫瑰杆菌。基因组和生化分析揭示了一些次级代谢物的潜力和产生，包括铁载体和与群体感应有关的酰基高丝氨酸内酯。在基因组中检测到了该色素的完整生物合成基因簇，质谱分析证实了该化合物的产生。此外，我们还研究了在共培养条件下，青霉的发光器共生体费氏弧菌和其他弧菌共培养条件下青黛的产生情况。最后，莱辛格氏菌(Leisingera sp.)该菌株的JC1和次生代谢物提取物对多种海洋弧菌具有不同的抗菌活性，表明莱辛格拉弧菌是一种典型的海洋弧菌。Jc1可能在卵和/或Ang中对其他海洋细菌起到宿主防御作用。这些数据还表明，这种与鱿鱼相关的细菌的抗菌活性可能部分是由吲哚定引起的，但不是全部。
Female members of many cephalopod species house a bacterial consortium in the accessory nidamental gland (ANG), part of the reproductive system. These bacteria are deposited into eggs that are then laid in the environment where they must develop unprotected from predation, pathogens, and fouling. In this study, we characterized the genome and secondary metabolite production of Leisingera sp. JC1, a member of the roseobacter clade (Rhodobacteraceae) of Alphaproteobacteria isolated from the jelly coat of eggs from the Hawaiian bobtail squid, Euprymna scolopes. Whole genome sequencing and MLSA analysis revealed that Leisingera sp. JC1 falls within a group of roseobacters associated with squid ANGs. Genome and biochemical analyses revealed the potential for and production of a number of secondary metabolites, including siderophores and acyl-homoserine lactones involved with quorum sensing. The complete biosynthetic gene cluster for the pigment indigoidine was detected in the genome and mass spectrometry confirmed the production of this compound. Furthermore, we investigated the production of indigoidine under co-culture conditions with Vibrio fischeri, the light organ symbiont of E. scolopes, and with other vibrios. Finally, both Leisingera sp. JC1 and secondary metabolite extracts of this strain had differential antimicrobial activity against a number of marine vibrios, suggesting that Leisingera sp. JC1 may play a role in host defense against other marine bacteria either in the eggs and/or ANG. These data also suggest that indigoidine may be partially, but not wholly, responsible for the antimicrobial activity of this squid-associated bacterium.
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影响因子：
4.4
作者：
Collins, Andrew J.;LaBarre, Brenna A.;Nyholm, Spencer V.
通讯作者：
Nyholm, Spencer V.
DOI：
10.1668/0003-1569(2001)041
发表时间：
2001-04-01
期刊：
AMERICAN ZOOLOGIST
影响因子：
--
作者：
Kramer, DL;McLaughlin, RL
通讯作者：
McLaughlin, RL
影响因子：
4.4
作者：
Bruhn, JB;Haagensen, JAJ;Gram, L
通讯作者：
Gram, L
影响因子：
3.9
作者：
Antonic, Vlado;Stojadinovic, Alexander;Alavi, Mohammad
通讯作者：
Alavi, Mohammad
影响因子：
3.2
作者：
Collins, Andrew J.;Nyholm, Spencer V.
通讯作者：
Nyholm, Spencer V.