Metaproteomic analysis of ratoon sugarcane rhizospheric soil.
Metaproteomic analysis of ratoon sugarcane rhizospheric soil.
复制标题
再生甘蔗根际土壤的宏蛋白质组学分析
DOI：
10.1186/1471-2180-13-135
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发表时间：
2013-06-17
期刊：
影响因子：
4.2
通讯作者：
Lin R
中科院分区：
文献类型：
--
作者：
Lin W;Wu L;Lin S;Zhang A;Zhou M;Lin R;Wang H;Chen J;Zhang Z;Lin R
背景本研究采用土壤元蛋白质组学技术结合群落水平生理谱（CLPP）分析方法，对宿根甘蔗产量下降的机理进行了研究。结果宿根甘蔗（RS）的有效茎数、茎粗、单茎重和理论产量显著低于种植甘蔗（NS）。RS土壤中转化酶、过氧化物酶、脲酶和磷酸单酯酶等几种碳、氮、磷加工酶的活性均显著低于NS土壤。生物多样性分析表明，与NS土壤相比，RS土壤的平均良好颜色发展（AWCD），Shannon的多样性和均匀度指数显着下降。为构建根际元蛋白质组图谱，成功鉴定了109个分辨率高、重复性好的土壤蛋白质点。这些蛋白质涉及碳水化合物/能量、氨基酸、蛋白质、核苷酸、生长素和次生代谢、膜转运、信号转导和抗性等方面。比较元蛋白质组学分析显示，与对照土壤或NS土壤相比，RS土壤中有38个蛋白质差异表达。其中，与碳水化合物和氨基酸代谢及胁迫响应相关的植物蛋白质在RS土壤中表达上调。此外，与膜运输和信号转导相关的几种微生物蛋白在RS土壤中表达上调。推测这些蛋白质的功能在根定植由microbiots.ConclusionsOur实验表明，甘蔗再生的做法引起显着的变化，土壤酶活性，微生物群落的分解代谢多样性，和土壤蛋白质的表达水平。它们影响着根际生态系统中的生物化学过程，并介导植物与土壤微生物之间的相互作用。
BackgroundThe current study was undertaken to elucidate the mechanism of yield decline in ratoon sugarcane using soil metaproteomics combined with community level physiological profiles (CLPP) analysis.ResultsThe available stalk number, stalk diameter, single stalk weight and theoretical yield of ratoon cane (RS) were found to be significantly lower than those of plant cane (NS). The activities of several carbon, nitrogen and phosphorus processing enzymes, including invertase, peroxidase, urease and phosphomonoesterase were found to be significantly lower in RS soil than in NS soil. BIOLOG analysis indicated a significant decline in average well-color development (AWCD), Shannon’s diversity and evenness indices in RS soil as compared to NS soil. To profile the rhizospheric metaproteome, 109 soil protein spots with high resolution and repeatability were successfully identified. These proteins were found to be involved in carbohydrate/energy, amino acid, protein, nucleotide, auxin and secondary metabolisms, membrane transport, signal transduction and resistance, etc. Comparative metaproteomics analysis revealed that 38 proteins were differentially expressed in the RS soil as compared to the control soil or NS soil. Among these, most of the plant proteins related to carbohydrate and amino acid metabolism and stress response were up-regulated in RS soil. Furthermore, several microbial proteins related to membrane transport and signal transduction were up-regulated in RS soil. These proteins were speculated to function in root colonization by microbes.ConclusionsOur experiments revealed that sugarcane ratooning practice induced significant changes in the soil enzyme activities, the catabolic diversity of microbial community, and the expression level of soil proteins. They influenced the biochemical processes in the rhizosphere ecosystem and mediated the interactions between plants and soil microbes.
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影响因子：
2.9
作者：
BLUM, H;BEIER, H;GROSS, HJ
通讯作者：
GROSS, HJ
影响因子：
3.7
作者：
Erickson AR;Cantarel BL;Lamendella R;Darzi Y;Mongodin EF;Pan C;Shah M;Halfvarson J;Tysk C;Henrissat B;Raes J;Verberkmoes NC;Fraser CM;Hettich RL;Jansson JK
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Jansson JK
影响因子：
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作者：
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通讯作者：
Aebersold, R
影响因子：
3.5
作者：
Dekkers, LC;Bloemendaal, CJP;Lugtenberg, BJJ
通讯作者：
Lugtenberg, BJJ
影响因子：
3.7
作者：
Chaparro JM;Badri DV;Bakker MG;Sugiyama A;Manter DK;Vivanco JM
通讯作者：
Vivanco JM