Metabolic Engineering of the Isopentenol Utilization Pathway Enhanced the Production of Terpenoids in Chlamydomonas reinhardtii.
Metabolic Engineering of the Isopentenol Utilization Pathway Enhanced the Production of Terpenoids in Chlamydomonas reinhardtii.
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异戊烯醇利用途径的代谢工程增强了莱茵衣藻中萜类化合物的产生
作者：
真核绿色微藻由于其快速、廉价的光养生长，在高价值精细化学品，特别是萜类化合物的可持续光驱动生物合成方面显示出相当大的前景。在这里，新的异戊烯醇利用途径（IUP）被引入到莱茵衣藻，以提高半萜（异戊烯焦磷酸，IPP）滴度。然后，二磷酸异构酶（IDI）和柠檬烯合酶（MsLS）进一步插入柠檬烯生产。与野生型相比，转基因藻类的IPP增加了8.6倍，与单一MsLS表达菌株相比，柠檬烯产量增加了23倍。在培养优化之后，当菌株在补充有10 mM异戊二烯醇的opt 2培养基中在光：暗方案下培养时，最高柠檬烯产量达到117 µg/L。这表明表达IUP的转基因藻类代表了高价值萜类化合物生产的理想底盘。IUP将进一步促进代谢和酶工程，以通过显著减少最佳生物合成所需的酶步骤的数量来提高萜类化合物滴度。
Eukaryotic green microalgae show considerable promise for the sustainable light-driven biosynthesis of high-value fine chemicals, especially terpenoids because of their fast and inexpensive phototrophic growth. Here, the novel isopentenol utilization pathway (IUP) was introduced into Chlamydomonas reinhardtii to enhance the hemiterpene (isopentenyl pyrophosphate, IPP) titers. Then, diphosphate isomerase (IDI) and limonene synthase (MsLS) were further inserted for limonene production. Transgenic algae showed 8.6-fold increase in IPP compared with the wild type, and 23-fold increase in limonene production compared with a single MsLS expressing strain. Following the culture optimization, the highest limonene production reached 117 µg/L, when the strain was cultured in a opt2 medium supplemented with 10 mM isoprenol under a light: dark regimen. This demonstrates that transgenic algae expressing the IUP represent an ideal chassis for the high-value terpenoid production. The IUP will facilitate further the metabolic and enzyme engineering to enhance the terpenoid titers by significantly reducing the number of enzyme steps required for an optimal biosynthesis.
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影响因子：
16.1
作者：
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5.2
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8.4
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Lauersen, Kyle J.;Baier, Thomas;Kruse, Olaf
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Kruse, Olaf